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Effect of induced peritoneal endometriosis on oocyte and embryo quality in a mouse model

  • Reproductive Physiology and Disease
  • Published:
Journal of Assisted Reproduction and Genetics Aims and scope Submit manuscript

Abstract

Purpose

To assess the impact of peritoneal endometriosis on oocyte and embryo quality in a mouse model.

Methods

Peritoneal endometriosis was surgically induced in 33 B6CBA/F1 female mice (endometriosis group, N = 17) and sham-operated were used as control (sham group, N = 16). Mice were superovulated 4 weeks after surgery and mated or not, to collect E0.5-embryos or MII-oocytes. Evaluation of oocyte and zygote quality was done by immunofluorescence under spinning disk confocal microscopy.

Results

Endometriosis-like lesions were observed in all mice of endometriosis group. In both groups, a similar mean number of MII oocytes per mouse was observed in non-mated mice (30.2 vs 32.6), with a lower proportion of normal oocytes in the endometriosis group (61 vs 83 %, p < 0.0001). Abnormalities were incomplete extrusion or division of the first polar body and spindle abnormalities. The mean number of zygotes per mouse was lower in the endometriosis group (21 vs 35.5, p = 0.02) without difference in embryo quality.

Conclusions

Our results support that induced peritoneal endometriosis in a mouse model is associated with a decrease in oocyte quality and embryo number. This experimental model allows further studies to understand mechanisms of endometriosis-associated infertility.

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Acknowledgments

We thank Romain Morichon for the use of the spinning disk confocal microscopy, Michele Oster for the histology, Lauriane Roche for her help in building the figures.

Author information

Authors and Affiliations

  1. Service de Gynécologie-Obstétrique, Hôpital Tenon, Assistance Publique des Hôpitaux de Paris, Université Pierre et Marie Curie Paris 6, GRC 6-UPMC Centre Expert en Endométriose (C3E), Paris, France

    J. Cohen, I. Naoura, N. Chabbert-Buffet & E. Darai

  2. Inserm UMRS938, Université Pierre et Marie Curie, Paris, France

    J. Cohen, I. Naoura, N. Chabbert-Buffet, S. Aractingi & E. Darai

  3. INSERM U1016, Institut Cochin, Université Paris Descartes, Paris, France

    A. Ziyyat & B. Lefevre

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  1. J. Cohen
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Correspondence to J. Cohen.

Additional information

Capsule In a mouse model, peritoneal endometriosis was responsible for decrease in oocyte quality and embryo quantity. Number of ovulated oocytes was not impaired.

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Cohen, J., Ziyyat, A., Naoura, I. et al. Effect of induced peritoneal endometriosis on oocyte and embryo quality in a mouse model. J Assist Reprod Genet 32, 263–270 (2015). https://doi.org/10.1007/s10815-014-0390-1

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  • DOI: https://doi.org/10.1007/s10815-014-0390-1

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