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Fluctuation of “sperm DNA integrity” in accordance with semen parameters, and it’s relationship with infertility

  • Assisted Reproduction Technologies
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Abstract

Purpose

Nowadays sperm analysis is used routinely to assess infertile men, but it’s predictive value is still restricted. For this reason, new markers are needed for diagnosis and guide treatment. A DNA damage evaluation method, which is easily applicable for routine analysis is described in this paper to provide an important support in clinical evaluation.

Methods

In this study, DNA damage evaluation was planned in infertile males with the COMET assay, a method which is simple, cheap, sensitive and reliable. For this purpose, the sperm DNA damage of normospermic, oligospermic, asthenospermic and teratospermic cases were investigated and the relationship between sperm parameters (sperm count, motility, morphology) and DNA damage was assessed.

Results and conclusion

According to our results we suggest that the factors to blame for sperm DNA damage and semen quality are the same. Also it was found that the infertile males possess substantially more sperm DNA damage than fertile men do. Improving and using the DNA damage evaluation tests seem to improve the results in ART clinics.

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Author information

Authors and Affiliations

  1. Faculty of Medicine, Family Planning-Infertility Research and Treatment Center, Ege University, Bornova, Izmir, Turkey

    Ayşen Durmaz, Ege Nazan Tavmergen Göker & Erol Tavmergen

  2. Faculty of Medicine, Department of Medical Biochemistry, Çukurova University, Adana, Turkey

    Nurten Dikmen

  3. Faculty of Medicine, Department of Medical Biology, Ege University, Izmir, Turkey

    Cumhur Gündüz

  4. Faculty of Medicine, Department of Obstetrics and Gynecology, Ege University, İzmir, Turkey

    Ege Nazan Tavmergen Göker & Erol Tavmergen

Authors
  1. Ayşen Durmaz
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  2. Nurten Dikmen
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  3. Cumhur Gündüz
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  4. Ege Nazan Tavmergen Göker
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  5. Erol Tavmergen
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Corresponding author

Correspondence to Ayşen Durmaz.

Additional information

Capsule An accurate sperm DNA damage analysis provides an important support in clinical evaluation so it is highly recommended before assisted reproductive technologies.

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Durmaz, A., Dikmen, N., Gündüz, C. et al. Fluctuation of “sperm DNA integrity” in accordance with semen parameters, and it’s relationship with infertility. J Assist Reprod Genet 31, 1665–1671 (2014). https://doi.org/10.1007/s10815-014-0364-3

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  • DOI: https://doi.org/10.1007/s10815-014-0364-3

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