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Effect of stem cell application on Asherman syndrome, an experimental rat model

  • Stem Cell Biology
  • Published:
Journal of Assisted Reproduction and Genetics Aims and scope Submit manuscript

Abstract

Purpose

We evaluate the effect of stem cells to induce endometrial proliferation and angiogenesis on Asherman Syndrome (AS).

Methods

The experimental study was performed in stemcell research laboratory. Forty Wistar-Albino rats were divided according to groups. In group1 (n = 10) to establish the model; trichloroacetic acid was injected to right uterine horn. Two weeks later, intrauterine synechia was confirmed. In group2 (n = 10), 2 weeks later, 2 × 106 mesenchymal stem cells (MSC) were injected into right uterine horn followed by three intraperitoneal injections of MSCs. In group3 (n = 10), daily oral estrogen was initiated on the second week. In group4 (n = 10), MSC injections and oral estrogen was given together. The amount of fibrosis, vascularisation, inflammation and immunohistochemical staining with vascular endothelial growth factor (VEGF), proliferating cell nuclear antigen (PCNA) and Ki-67 were evaluated in the uterine tissues.

Results

In all treatment groups; fibrosis decreased but vascularisation and immunhistohemical stainings increased in the experimental side. The amount of fibrosis, vascularisation, Ki-67 and PCNA scores were similar between group2 and 3. In group4, comparing to group2, less fibrosis but more Ki-67, PCNA and VEGF staining was observed.

Conclusion

Stem cells, when added to estrogen, are a highly effective alternative to induce regeneration of endometrium in Asherman Syndrome therapy.

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Authors and Affiliations

  1. Kastamonu School of Medicine, Hacettepe University, Süleyman bey sokak 29/10, Maltepe, Ankara, 06420, Turkey

    Sevtap Kilic

  2. Department of Assisted Reproductive Technologies, Dumlupinar University School of Medicine, 3222. Cadde 9b/12, Yasamkent, Ankara, Turkey

    Beril Yuksel

  3. Yildirim Beyazit Research Hospital, Adacell Laboratory, Pancreas Islet Cell Research Center, Ankara, Turkey

    F. Pinarli, B. Boztok & T. Delibasi

  4. Pathology Department of Numune Research and Educational Hospital, Istanbul, Turkey

    A. Albayrak

Authors
  1. Sevtap Kilic
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  2. Beril Yuksel
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  3. F. Pinarli
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  4. A. Albayrak
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  5. B. Boztok
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  6. T. Delibasi
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Corresponding author

Correspondence to Sevtap Kilic.

Additional information

Capsule Stem Cell Application for Uterine Synechia

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Kilic, S., Yuksel, B., Pinarli, F. et al. Effect of stem cell application on Asherman syndrome, an experimental rat model. J Assist Reprod Genet 31, 975–982 (2014). https://doi.org/10.1007/s10815-014-0268-2

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  • DOI: https://doi.org/10.1007/s10815-014-0268-2

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