Gamete Biology

Journal of Assisted Reproduction and Genetics

, Volume 28, Issue 6, pp 531-537

In vitro maturation of ovine oocytes using different maturation media: effect of human menopausal serum

  • H. Karami ShabankarehAffiliated withDepartment of Animal Science, Razi University Email author 
  • , K. SarsaifiAffiliated withAnimal Reproduction Physiology, Department of Animal Science, Razi University
  • , T. MehranniaAffiliated withDepartment of Anatomy, Medical Faculty, University of Medical Science

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Abstract

Objective

To compare the effect of human menopausal serum with estrous sheep serum, estrous goat serum, ovine follicular fluid and bovine follicular fluid on in vitro maturation, in vitro fertilization and embryo development of sheep oocytes

Method (s)

Oocytes were treated in culture with different sera and follicular fluids supplemented media to examine effects on embryo development.

Results

Basic culture medium supplemented with human menopausal serum, estrous sheep serum and estrous goat serum supported better rates of in vitro maturation, in vitro fertilization and embryo development. Ovine follicular fluid and bovine follicular fluid supplementations supported similar rates of In vitro maturation, In vitro fertilization and embryo development which were lower than those supported by human menopausal serum, estrous sheep serum, estrous goat serum and control medium.

Conclusion

Human menopausal serum, estrous sheep serum, and estrous goat serum resulted in higher maturation, fertilization and embryo development than ovine follicular fluid, bovine follicular fluid and control media.

Keywords

Human menopausal serum Ovine Oocyte maturation