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Heparin-binding epidermal growth factor (HB-EGF) may improve embryonic development and implantation by increasing vitronectin receptor (integrin ανβ3) expression in peri-implantation mouse embryos

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Purpose: This study investigated the effects of HB-EGF on expression of integrin \(\alpha\nu\beta\hbox{{\it 3}}\) and implantation of embryos.

Methods: Two-cell embryos were recovered and cultured with or without 10 ng/mL HB-EGF for 96h. Expression of integrin \(\alpha\nu\beta\hbox{{\it 3}}\) in cultured embryos was examined by real time-RT-PCR and immunofluorescence analysis; embryos were cultured with or without HB-EGF, then transferred into the uteri of pseudo-pregnant female mice in order to analyze their implantation rate.

Results: HB-EGF improved embryonic hatching and outgrowth during extended culture, and up-regulated expression of integrin \(\alpha\nu\beta\hbox{{\it 3}}\) in both the preimplantation embryo and outgrowing blastocyst. Also, integrin \(\alpha\nu\beta\hbox{{\it 3}}\) subunits were localized at the pericellular borders and cell–cell contact areas. The number of successful implantation sites of transferred HB-EGF-treated embryos in the uterus was increased when compared to number of implantation sites with non-treated controls.

Conclusions: HB-EGF may improve implantation by accelerating expression of integrin \(\alpha\nu\beta\hbox{{\it 3}}\) in peri-implantation mouse embryos.

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Authors and Affiliations

  1. Department of Life Science, College of Natural Science, Hanyang University, 17 Haengdang-dong, Seongdong-gu, Seoul, 133-791, Korea

    Jung Jin Lim, Haeng-Seok Song, Myung Chan Gye & Moon Kyoo Kim

  2. Fertility Center of CHA General Hospital, CHA Research Institute, Pochon CHA University College of Medicine, 606-5 Yeoksam-dong, Gangnam-gu, Seoul, 135-081, Korea

    Jung Jin Lim, Dong Ryul Lee & Tae Ki Yoon

  3. Department of Anatomy and Cell Biology, College of Medicine, Hanyang University, 17 Haengdang-dong, Seongdong-gu, Seoul, 133-791, Korea

    Jung Jin Lim & Kye-Seong Kim

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  1. Jung Jin Lim
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  2. Dong Ryul Lee
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  3. Haeng-Seok Song
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Correspondence to Moon Kyoo Kim.

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J.J. Lim and D.R. Lee contributed equally to this work.

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Lim, J.J., Lee, D.R., Song, HS. et al. Heparin-binding epidermal growth factor (HB-EGF) may improve embryonic development and implantation by increasing vitronectin receptor (integrin ανβ3) expression in peri-implantation mouse embryos. J Assist Reprod Genet 23, 111–119 (2006). https://doi.org/10.1007/s10815-006-9021-9

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  • DOI: https://doi.org/10.1007/s10815-006-9021-9

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