A dual wavelength UV-spectrophotometric method has been developed for the simultaneous estimation of mycophenolate mofetil and prednisolone. In this method two wavelengths were selected for the estimation of each drug in such a way that the difference in the absorbance was zero for the second drug on the respective wavelength for the first drug. This method was selected because of the overlapping of the absorbance maxima of the drugs. Prednisolone has equal absorbance value at wavelengths 235.11 and 261.33 nm; therefore, these two wavelengths were used to determine the concentration of mycophenolate mofetil in the combination. Similarly, 270.3 and 277.4 nm wavelengths were selected to determine the concentrations of prednisolone, where mycophenolate mofetil was observed with equal absorbance values. Regression analysis for the method shows good correlation in the concentration ranges 10–50 μg/ml for mycophenolate mofetil and 2–10 μg/ml for prednisolone. The method was validated using parameters provided as per ICH guidelines.
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Published in Zhurnal Prikladnoi Spektroskopii, Vol. 81, No. 3, pp. 408–412, May–June, 2014.
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Kaur, J., Sharma, P.K., Namdev, K.K. et al. Development and Validation of a Novel Dual Wavelength UV-Spectrophotometric Method for the Simultaneous Estimation of Mycophenolate Mofetil and Prednisolone. J Appl Spectrosc 81, 427–431 (2014). https://doi.org/10.1007/s10812-014-9949-y
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DOI: https://doi.org/10.1007/s10812-014-9949-y