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Applying the strategy of light environment control to improve the biomass and polysaccharide production of Nostoc flagelliforme

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Abstract

The strategy of light environment control was applied to improve biomass and extracellular polysaccharides (EPS) production of the cyanobacterium Nostoc flagelliforme by adjusting multiple wavelengths (red 660 nm, blue 460 nm, green 520 nm) and light intensity assisted by nitrogen source optimization. A mixed wavelength with low light intensity was more suitable for cell growth. Wavelength shift approach, i.e., manipulation of light wavelength at appropriate culture stages, increased both biomass and EPS production, and optimum shift time was at 9 days. The effects of four nitrogen sources under different light conditions were subsequently evaluated, and urea showed the best performance. The optimized wavelength shift approach (9-day illumination with white light followed by 9-day culture with mixed wavelengths of red/blue/green = 12:5:5) with urea as nitrogen source improved the biomass from 0.72 ± 0.02 to 1.20 ± 0.02 g L−1 (i.e., by 66 %) and EPS production from 27.31 ± 1.00 to 86.65 ± 2.56 mg L−1 (i.e., by 217.3 %). These results provide information on novel culture strategies for microalgal biotechnology by applying light environment control.

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Acknowledgments

The authors are very grateful for the financial support from the National Natural Science Foundation of China (Grant Nos. 31671842 and 31201405) and Changjiang Scholars and Innovative Research Team in University (Grant No. IRT1166).

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Authors and Affiliations

  1. Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, School of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, China

    Pei-pei Han, Shi-gang Shen, Hui-Yan Wang, Shun-yu Yao, Zhi-lei Tan, Cheng Zhong & Shi-ru Jia

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  1. Pei-pei Han
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  2. Shi-gang Shen
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  3. Hui-Yan Wang
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  4. Shun-yu Yao
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  5. Zhi-lei Tan
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  6. Cheng Zhong
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  7. Shi-ru Jia
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Correspondence to Shi-ru Jia.

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Han, Pp., Shen, Sg., Wang, HY. et al. Applying the strategy of light environment control to improve the biomass and polysaccharide production of Nostoc flagelliforme . J Appl Phycol 29, 55–65 (2017). https://doi.org/10.1007/s10811-016-0963-8

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  • DOI: https://doi.org/10.1007/s10811-016-0963-8

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