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Timing is everything: optimizing crop yield for Thalassiosira pseudonana (Bacillariophyceae) with semi-continuous culture

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Abstract

There is relatively little choice in cultivation methods for growing algae outdoors, either in open pond systems or closed photobioreactors—as batch, continuous, or semi-continuous culture. Algal batch culture grown in a nutrient replete environment with adequate sunlight will become self-shaded with sufficient cell density and enter a stage in the growth dynamic known as the “phase of linear growth.” It is during this phase of linear growth that primary production is at maximum and that the highest biomass is harvested. The inherent problem with batch culture is that the exponential (and possibly lag) phases necessary to achieve densities required prior to the phase of linear growth consume time and waste surface area, and thereby make this an inefficient method to grow algae. Semi-continuous culture can be forced into shade-limiting conditions by reducing growth rate from maximum through dilution, whereby phases of lag and exponential growth are skipped, and culture growth is put into a state similar to a perpetual phase of linear growth with an appropriate culture harvest/dilution cycle. Importantly, semi-continuous culture can increase net growth efficiency over batch culture when compared by shade-limited growth rate. However, scientific study and theory covering shade-limited algal growth under semi-continuous culture conditions are nearly non-existent, which currently makes its application to phycological technologies impractical through “hit and miss” strategies. This laboratory study compares shade-limited growth dynamics for batch and semi-continuous cultures of Thalassiosira pseudonana (small-sized, marine diatom). Theory for optimizing production of mass algal culture with semi-continuous culture technique through cycle period and harvest volume is developed, and guidelines to practical industrial applications are provided.

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References

  • Béchet Q, Shilton A, Guieysse B (2013) Modeling the effects of light and temperature on algae growth: state of the art and critical assessment for productivity prediction during outdoor cultivation. Biotech Adv 31:1648–1663

    Article  Google Scholar 

  • Brand LE, Guillard RL (1981) The effects of continuous light and light intensity on the reproduction rate of twenty-two species of marine phytoplankton J Exper Mar Biol Ecol 50:119–132

  • Chen C, Durbin EG (1994) Effects of pH on the growth and carbon uptake of marine phytoplankton. Mar Ecol Prog Ser 109:83–94

    Article  Google Scholar 

  • Doucha J, Lívanský K (2009) Outdoor open thin-layer microalgal photobioreactor: potential productivity. J Appl Phycol 21:111–117

    Article  CAS  Google Scholar 

  • Droop MR (1968) Vitamin B12 and marine ecology. IV. The kinetics of uptake, growth and inhibition in Monochrysis lutheri. J Mar Biol Assoc 48:689–733

    Article  CAS  Google Scholar 

  • Eppley RW (1972) Temperature and phytoplankton growth in the sea. Fish Bull 70:1063–1085

    Google Scholar 

  • Eppley RW, Dyer DL (1965) Predicting production in light-limited continuous cultures of algae. App Microbiol 13:833–837

    CAS  Google Scholar 

  • Ferguson RL, Collier A, Meeter DA (1976) Growth response of Thalassiosira pseudonana Hasle and Heimdal clone 3H to illumination, temperature and nitrogen source. Chesap Sci 17:148–158

    Article  Google Scholar 

  • Goldman JC (1979) Outdoor algal mass cultures—II. Photosynthetic yield limitations. Water Res 13:119–136

    Article  CAS  Google Scholar 

  • Hewes CD (2015a) Transitional-state growth kinetics of Thalassiosira pseudonana (Bacillariophyceae) during self-shading in batch culture under light-limiting, nutrient-replete conditions: Improving biomass for productivity (culture quality). Algal Res 12:550–560

    Article  Google Scholar 

  • Hewes CD (2015b) Not all culture is created equal: a comparative study in search of the most productive cultivation methodology. Algal Res 12:561–568

    Article  Google Scholar 

  • Hewes CD, Hewes SO (2014) SolarStatTM: An incubator system for the study of shade-limited algal cultures in fluctuating light, UCSD Reference No. 2015-022 http://techtransfer.universityofcalifornia.edu/NCD/24393.html

  • Hewes CD, Hewes SO (2016) First steps toward standardizing dynamic light regimes for the quantitative study of light-controlled growth in shade-limiting water columns. J Appl Phycol. doi:10.1007/s10811-016-0901-9

  • Hoekema S, Rinzema A, Tramper T, Wijffels RH, Janssen M (2014) Deceleration-stats save much time during phototrophic culture optimization. Biotech Bioeng 111:792–802

    Article  CAS  Google Scholar 

  • Ketchum BH, Lillick L, Redfield AC (1949) The growth and optimum yields of unicellular algae in mass culture. J Cell Comp Physiol 33:267–279

    Article  CAS  Google Scholar 

  • Laws EA, Taguchii S, Hirata J, Pang L (1986a) High algal production rates achieved in a shallow outdoor flume. Biotechnol Bioeng 28:191–197

    Article  CAS  PubMed  Google Scholar 

  • Laws EA, Taguchii S, Hirata J, Pang L (1986b) Continued studies of high algal productivities in a shallow flume. Biomass 11:39–50

    Article  Google Scholar 

  • Lundquist TJ, Woertz IC, Quinn NWT, Benemann JR (2010) A realistic technology and engineering assessment of algal biofuel production. Energy Biosciences Institute, Berkley

    Google Scholar 

  • MacIntyre H, Kana TM, Anning T, Geider RJ (2002) Photoacclimation of photosynthesis irradiance response curves and photosynthetic pigments in microalgae and cyanobacteria. J Phycol 38:17–38

    Article  Google Scholar 

  • McBride RC, Lopez S, Meenach C, Burnett M, Lee PA, Nohilly F, Behnke C (2014) Contamination management in low cost open algae ponds for biofuels production. Ind Biotech 10:221–227

    Article  Google Scholar 

  • Monod J (1950) La technique de la culture continue: theorie et applications. Ann Inst Pasteur, Lille 79:390–410

    CAS  Google Scholar 

  • Nelson DM, D’Elia CF, Guillard RRL (1979) Growth and competition of the marine diatoms Phaeodactylum tricornutum and Thalassiosira pseudonana II. Light limitation Mar Biol 50:313–318

    Article  Google Scholar 

  • Panikov NA (1995) Microbial growth kinetics. Chapman and Hall, London

    Google Scholar 

  • Richmond A (1986) Outdoor mass cultures of microalgae. In: Richmond A (ed) Handbook of microalgal mass culture. CRC Press, Boca Raton, pp 285–329

    Google Scholar 

  • Richmond A (2004) Biological principles of mass cultivation. In: Richmond A (ed) Handbook of microalgal culture biotechnology and applied phycology. Blackwell Science, Singapore, pp 125–177

    Google Scholar 

  • Sheehan J, Dunahay T, Beneman J, Roessler PG (1998) U.S. Department of Energy’s Office of Fuels Development: a look back at the U.S. Department of Energy’s Aquatic Species Program- Biodiesel from algae, Golden, CO: National Renewable Energy Laboratory, NREL/TP-580-24190

  • Thompson P (1999) The response of growth and biochemical composition to variations in daylength, temperature, and irradiance in the marine diatom Thalassiosira pseudonana (Bacillariophyceae). J Phycol 35:1215–1223

    Article  CAS  Google Scholar 

  • Weissman JC, Benemann J (1979) Biomass recycling and species competition in continuous cultures. Biotech Bioeng 21:627–648

    Article  Google Scholar 

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Acknowledgments

Thanks to Oz Holm-Hansen, who provided space and equipment to perform this study, and to Dom Mendola who previewed this MS and offered suggestions. Great appreciation is given to the Marine Biology Research Division at SIO, whose researchers and staff offered an enjoyable and stimulating working environment. Thanks also to the anonymous reviewers and editor who helped improve this manuscript. No government agency can claim economic support for the experimental and reporting efforts of the project as described in this manuscript. Views expressed herein are those solely of the author, and there are no conflicts of interest.

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Correspondence to Christopher D. Hewes.

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Hewes, C.D. Timing is everything: optimizing crop yield for Thalassiosira pseudonana (Bacillariophyceae) with semi-continuous culture. J Appl Phycol 28, 3213–3223 (2016). https://doi.org/10.1007/s10811-016-0900-x

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  • DOI: https://doi.org/10.1007/s10811-016-0900-x

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