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Isolation and identification of anti-inflammatory compounds from ethyl acetate fraction of Ecklonia stolonifera and their anti-inflammatory action

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Abstract

Ecklonia stolonifera, a brown alga, has been reported to have several biological activities, including antioxidation, anti-inflammation, and hepatoprotection. This study aims to investigate the anti-inflammatory effect of the ethyl acetate fraction of E. stolonifera (ESA) in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. ESA inhibited the production of LPS-induced nitric oxide (NO) and prostaglandin E2 and reduced the expression of inducible nitric oxide synthase and cyclooxygenase-2 in a dose-dependent manner. ESA also inhibited the production of pro-inflammatory cytokines, including tumor necrosis factor α, interleukin (IL)-1β, and IL-6. LPS-induced transcriptional activation of nuclear factor-κB was significantly suppressed by ESA through the prevention of inhibitor κB-α degradation. Eight phlorotannins, including 2-phloroeckol, dioxinodehydroeckol, eckol, phlorofucofuroeckol B, 6,6′-bieckol, dieckol, 974-B, and phlorofucofuroeckol A were isolated from ESA, and their chemical structures were elucidated by NMR spectroscopic analysis. Based on the inhibition of NO production in LPS-treated RAW 264.7 cells, the major anti-inflammatory components in ESA were identified as 2′-phloroeckol, 6,6′-bieckol, phlorofucofuroeckol A, phlorofucofuroeckol B, and 974-B. Our results indicate that E. stolonifera may be considered as a therapeutic agent for the prevention of inflammatory disorders.

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Acknowledgments

This research was supported by the project “Development of nutraceuticals from Sargassum serratifolium” funded by the Ministry of Oceans and Fisheries, Korea.

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Authors and Affiliations

  1. Department of Food Science and Nutrition, Pukyong National University, 599-1, Daeyeon 3-dong, Nam-gu, Busan, 608-737, South Korea

    Ran Wei, Min-Sup Lee & Hyeung-Rak Kim

  2. College of Pharmacy, Pusan National University, Gumjung-gu, Busan, 619-705, South Korea

    Bonggi Lee

  3. Department of Marine Biology, Pukyong National University, Busan, 608-737, South Korea

    Chul-Woong Oh

  4. Department of Ecological Engineering, Pukyong National University, Busan, 608-737, South Korea

    Chang-Geun Choi

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  1. Ran Wei
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  2. Min-Sup Lee
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Correspondence to Hyeung-Rak Kim.

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Wei, R., Lee, MS., Lee, B. et al. Isolation and identification of anti-inflammatory compounds from ethyl acetate fraction of Ecklonia stolonifera and their anti-inflammatory action. J Appl Phycol 28, 3535–3545 (2016). https://doi.org/10.1007/s10811-016-0847-y

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