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Maternal inheritance of organellar DNA demonstrated with DNA markers in crosses of Saccharina japonica (Laminariales, Phaeophyta)

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Abstract

Two simple sequence repeats from chloroplast DNA (cpSSRs) and two mitochondrial DNA markers (rpl6-rps2 and trnW-trnI) were developed and used to investigate inheritance patterns of chloroplast and mitochondrial DNA in Saccharina japonica. Haplotypes were tested in 76 progeny sporophytes from two sets of reciprocal crosses between male and female gametophytes of S. japonica. The haplotypes were consistent with those of the maternal parents, proving that both the chloroplast and mitochondrial genomes were maternally inherited in all crosses. Forty-two cpSSRs and fourteen mtSSRs markers were developed for the present study, of which two cpSSRs and two mtSSRs proved to be polymorphic in 24 S. japonica individuals.

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Acknowledgments

This study was supported by the National Science Foundation of China (31272660), the National Key Technology Research and Development Program (2013BAB01B01), and the Ocean Public Welfare Scientific Research Project (201405040). We thank Donald W. Sturge and Janet W. Reid for revising the English text, and acknowledge anonymous reviewers for their critical comments and valuable suggestions.

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Authors and Affiliations

  1. Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China

    Qiuying Li, Xiuliang Wang, Jie Zhang, Jianting Yao & Delin Duan

  2. University of Chinese Academy of Sciences, Beijing, 100049, China

    Qiuying Li & Jie Zhang

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  1. Qiuying Li
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  2. Xiuliang Wang
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  3. Jie Zhang
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  4. Jianting Yao
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  5. Delin Duan
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Correspondence to Xiuliang Wang or Delin Duan.

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Li, Q., Wang, X., Zhang, J. et al. Maternal inheritance of organellar DNA demonstrated with DNA markers in crosses of Saccharina japonica (Laminariales, Phaeophyta). J Appl Phycol 28, 2019–2026 (2016). https://doi.org/10.1007/s10811-015-0687-1

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  • DOI: https://doi.org/10.1007/s10811-015-0687-1

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