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Biochemical characterization of Pyropia yezoensis-AP1 strain accompanies the resistance reaction to the red rot disease pathogen, Pythium porphyrae

  • 5th Congress of the International Society for Applied Phycology
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Abstract

The biochemical profile of the Pyropia yezoensis-AP1 strain, a strain which shows resistance to red rot disease, was compared with that of a normal P. yezoensis cultivar. Levels of chlorophyll a, phycoerythrin, and phycocyanin were higher in P. yezoensis-AP1 than those in the normal P. yezoensis cultivar. Two-dimensional electrophoresis found a total of 132 and 128 distinct spots for total protein samples of P. yezoensis-AP1 and P. yezoensis, respectively. About 89.6 % of the total components were common to both P. yezoensis-AP1 and P. yezoensis, and 10.4 % were more abundant in the P. yezoensis-AP1 than P. yezoensis. The amount of total free amino acid in P. yezoensis-AP1 was less than that in the P. yezoensis cultivar, but taurine, glutamic acid, citrulline, and arginine content were higher in the P. yezoensis-AP1 strain. The porphyran content of P. yezoensis-AP1 (11.9 ± 0.5 % dry weight) was higher than that of P. yezoensis cultivar (9.5 ± 0.4 % dry weight). In the porphyran fraction, the content of 3,6-anhydro-L-galactose was higher in P. yezoensis-AP1 than P. yezoensis, and sulfate content was higher in the P. yezoensis cultivar than P. yezoensis-AP1. The results indicate that the difference of chemical composition between P. yezoensis-AP1 and P. yezoensis cultivar helps to explain the increased resistance to red rot disease in the AP1 strain. This finding provides a good starting point for the development of more resistant strains of P. yezoensis that will reduce the incidence of red rot disease on Pyropia farms.

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Acknowledgments

This research was partly supported by Priority Research Centers Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2009–0093828), and NFRDI (RP-2014-AQ-061). The authors would like to thank Dr Philip Heath (New Zealand) for reviewing the English.

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Authors and Affiliations

  1. Department of Marine and Fisheries Resources, Mokpo National University, Muan-gun, Jeonnam, 534-729, Korea

    Chan Sun Park

  2. Seaweed Research Center, National Fisheries Research and Development Institute, 130 Tongildaero, Mokpo, Jeonnam, 530-831, Korea

    Eun Kyoung Hwang

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  1. Chan Sun Park
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  2. Eun Kyoung Hwang
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Correspondence to Eun Kyoung Hwang.

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Park, C.S., Hwang, E.K. Biochemical characterization of Pyropia yezoensis-AP1 strain accompanies the resistance reaction to the red rot disease pathogen, Pythium porphyrae . J Appl Phycol 27, 2149–2156 (2015). https://doi.org/10.1007/s10811-015-0527-3

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  • DOI: https://doi.org/10.1007/s10811-015-0527-3

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