Abstract
Determining the fraction of viable cells in algal cultures is critical to improve the understanding and control of algal microbiology, ecology, and biotechnology. Whereas current techniques for algal viability determination can be rather cumbersome, this paper describes a new assay that enables the rapid quantification of algal viability using only spectrophotometric measurements. This technique, henceforth named colorimetric assay of viability for algae (CAVA), relies on the selective adsorption of erythrosine by non-viable cells and was validated on the algae species Chlamydomonas reinhardtii and Chlorella vulgaris. The results obtained by the CAVA test were in good agreement with the in situ measurement of oxygen production rates. In addition, the CAVA test was shown to quantify the viability of algal samples regardless of the cause of death (heating, UV irradiation, or H2O2 exposure). The CAVA technique has therefore the potential to offer a fast and universal approach to measure the viability of algal samples.
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Acknowledgments
Dr. Mike Packer, Cawthron Institute (New Zealand), is kindly acknowledged for providing axenic cultures of C. reinhardtii. Cyril Breton, Ecole Centrale Paris (France), and Laura Etienne, Massey University (New Zealand), are also acknowledged for preliminary experimental work during the technique development. Maxence Plouviez, Massey University (New Zealand), is also thanked for his help all along the project.
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Béchet, Q., Feurgard, I., Guieysse, B. et al. The colorimetric assay of viability for algae (CAVA): a fast and accurate technique. J Appl Phycol 27, 2289–2297 (2015). https://doi.org/10.1007/s10811-014-0508-y
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DOI: https://doi.org/10.1007/s10811-014-0508-y