Abstract
Traditionally Blumea lacera DC is used to treat inflammation and bowel ailments. Lack of specific, curative treatment for IBD enticed us to investigate the therapeutic efficacy of ethanolic extract of aerial parts of Blumea lacera DC (EEBL) against indomethacin-induced enterocolitis. Male Wistar rats were divided into six groups (n = 5) and different doses of EEBL (100 and 200 mg/kg, p.o) and sulphasalazine (100 mg/kg, p.o) were administered for seven days. Enterocolitis was induced by two subsequent doses of indomethacin (7.5 mg/kg, s.c) on 7th and 8th day. Treatments were continued up to 12th day and sacrificed. The protective effect was assessed on the basis of macroscopic scores of ileum strips, changes in biochemical parameters such as serum lactate dehydrogenase (LDH), tissue myeloperoxidase (MPO), lipid peroxidation (LPO), and total thiols (TT). Further, activity was ascertained by histopathological evaluations. HPLC fingerprinting profiling of EEBL was also carried out. Pre-treatment with EEBL or sulphasalazine significantly attenuated the indomethacin-induced proximal ileal damage, elevated levels of serum LDH, tissue MPO, LPO and lower levels of TT. Further, observed activity of EEBL was well correlated with histopathological alterations. The results revealed the protective action of the title plant against the indomethacin-induced enterocolitis in rats, which might be attributed by its antioxidant, anti-inflammatory, antimicrobial, and membrane-stabilizing properties.
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References
Abraham C, Cho JH (2009) Inflammatory bowel disease. N Engl J Med 361(21):2066–2078
Al-Rejaie SS, Abuohashish HM, Al-Enazi MM, Ak-Assaf AH, Parmar MY, Ahmed MM (2013) Protective effect of naringenin on acetic acid-induced ulcerative colitis in rats. World J Gastroenterol 19(34):5633–5644
Amarowich R (2007) Tannins: the new natural antioxidants? Eur J Lipid Sci Technol 109(6):549–551
Dickinson DA, Forman HJ (2002) Cellular glutathione and thiols metabolism. Biochem Pharmacol 64(5–6):1019–1026
Elson CO, Sartor RB, Tennyson GS, Riddell RH (1995) Experimental models of inflammatory bowel disease. Gastroenterology 109(4):1344–1367
Hagar HH, El-Medany A, El-Eter E, Arafa M (2007) Ameliorative effect of pyrrolidinedithiocarbamate on acetic acid-induced colitis in rats. Eur J Pharmacol 554(1):69–77
Harputluoglu MM, Demirel U, Yucel N, Karadag N, Temel I, Firat S, Ara C, Aladag M, Karincaoglu M, Hilmioglu F (2006) The effects if Gingko biloba extract on acetic acid-induced colitis in rats. Turk J Gastroenterol 17(3):177–182
Head KA, Jurenka JS (2003) inflammatory bowel disease part 1: ulcerative colitis—pathophysiology and conventional and alternative treatment options. Altern Med Rev 8(3):247–283
Khair A, Ibrahm M, Ahsan Q, Homa Z, Kuddus MR, Rashid RB, Rashid MA (2014) Pharmacological activities of Blumea lacera (Burm.f.) DC: a medicinal plant of Bangladesh. Br. J Pharm Res 4(13):1677–1687
Khandekar U, Tippat S, Hongade R (2013) Inverstigation on antioxidant, antimicrobial and phytochemical profile of Blumea lacera leaf. Int J Biol Pharm Res 4(11):756–761
Kumar A (2010) Medicinal plants. International Scientific Publishing Academy, New Delhi
Lim YJ, Yang CH (2012) Non-steroidal anti-inflammatory drug-induced enteropathy. Clin Endosc 45(2):138–144
Meister A (1988) Glutathione mechanism and its selective modification. J Biol Chem 263(33):17205–17208
Navarro F, Hanauer SB (2003) Treatment of inflammatory bowel disease: safety and tolerability issues. Amm J Gastroenterol 98(Suppl 12):S18–S23
Othman RA, Moghadasian MH (2011) Beyond cholesterol-lowering effects of plant sterols:clinical and experimental evidence of anti-inflammatory properties. Nutr Rev 69(7):371–382
Pawar AT, Anap RM, Ghodasara JV, Kuchekar BS (2011) Protective effect of hydroalcoholic root extract of Rubia cordifolia in indomethacin-induced enterocolitis in rats. Ind J Pharm Sci 73(2):250–253
Pietta PG (2000) Flavonoids as antioxidants. J Nat Prod 63(7):1035–1042
Ponder A, Long MD (2013) A clinical review of recent findings in the epidemiology of inflammatory bowel disease. Clin Epidemiol 5:237–247
Rahman A, Bhuiyan MA, Shahriar M (2013) Preliminary in vitro biological, phytochemical screenings and in vitro antioxidant activities of Blumea lacera (Burm.f.) DC. Int Arch Appl Sci Technol 4(2):1–7
Sedlak J, Lindsay RH (1968) Estimation of total, protein bound, and nonprotein sulfahydryl groups in tissue with ellman’s reagent. Anal Biochem 25(1):192–205
Stadnicki A, Colman RW (2003) Experimental models of inflammatory bowel disease. Arch Immunol Ther Exp (Warsz) 51(3):149–155
Takeuchi K, Tanaka A, Ohno R, Yokota A (2003) Role of COX inhibition in pathogenesis of NSAID-induced small intestinal damage. J Physiol Pharmacol 54(Suppl 4):165–182
Tanaka A, Hase S, Miyazawa T, Ohno R, Takeuchi K (2002) Role of cyclooxygenase (COX)-1 and COX-2 inhibition in nonsteroidal anti-inflammatory drug-induced intestinal damage in rats: relation to various pathogenic events. J Pharmacol Exp Ther 303(3):1248–1254
Thippeswamy BS, Mahendran S, Biradar MI, Raj P, Srivastava K, Badami S, Veerapur VP (2011) Protective effect of embelin against acetic acid induced ulcerative colitis in rats. Eur J Pharmacol 654(1):100–105
Yamada T, Deitch E, Specian RD, Perry MA, Sartor RB, Grisham MB (1993) Mechanisms of acute and chronic intestinal inflammation induced by indomethacin. Inflammation 17(6):641–662
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Basnet, S., Adhikari, A., Sachidananda, V.K. et al. Protective effect of Blumea lacera DC aerial parts in indomethacin-induced enterocolitis in rats. Inflammopharmacol 23, 355–363 (2015). https://doi.org/10.1007/s10787-015-0243-2
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DOI: https://doi.org/10.1007/s10787-015-0243-2