Research Article

Inflammopharmacology

, Volume 19, Issue 2, pp 99-110

Anti-inflammatory properties of Doxycycline and Minocycline in experimental models: an in vivo and in vitro comparative study

  • Liz M. LeiteAffiliated withFaculty of Medicine of Juazeiro de Norte
  • , Antônio Germano G. CarvalhoAffiliated withFaculty of Medicine of Juazeiro de Norte
  • , Pollyanna L. F. Tavares FerreiraAffiliated withFaculty of Medicine of Juazeiro de Norte
  • , Igor Xavier PessoaAffiliated withFaculty of Medicine of Juazeiro de Norte
  • , Danilo O. GonçalvesAffiliated withDepartment of Physiology and Pharmacology, Federal University of Ceará
  • , Amanda de Araújo LopesAffiliated withDepartment of Physiology and Pharmacology, Federal University of Ceará
  • , Jean Guilherme dos Santos GóesAffiliated withDepartment of Physiology and Pharmacology, Federal University of Ceará
  • , Victor Costa de Castro AlvesAffiliated withDepartment of Physiology and Pharmacology, Federal University of Ceará
  • , Luzia Kalyne A. M. LealAffiliated withDepartment of Physiology and Pharmacology, Federal University of Ceará
    • , Gerly Anne BritoAffiliated withFaculty of Medicine of Juazeiro de NorteDepartment of Physiology and Pharmacology, Federal University of Ceará
    • , Glauce S. B. VianaAffiliated withFaculty of Medicine of Juazeiro de NorteDepartment of Physiology and Pharmacology, Federal University of CearáRua Barbosa de Freitas Email author 

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Abstract

Aims and methods

Minocycline (Mino) and doxycycline (Dox) are second generation tetracyclines known to present several other effects, which are independent from their antimicrobial activities. We studied in a comparative way the anti-inflammatory effects of Mino and Dox, on acute models of peripheral inflammation in rodents (formalin test and peritonitis in mice, and carrageenan-induced paw oedema in rats). Immunohistochemical assays for TNF-alpha and iNOS in rat paws of carrageenan-induced oedema were also carried out as well as in vitro assays for myeloperoxidase (MPO) and lactate dehydrogenase (LDH). Furthermore, antioxidant activities were evaluated by the DPPH assay.

Results

In the formalin test although Mino and Dox (1, 5, 10 and 25 mg/kg, i.p.) inhibited the first phase, they acted predominantly on the second phase of the test, where inhibition of the licking time close to 80% were observed. Mino and Dox were very efficacious in reducing the carrageenan-induced paw oedema in rats (10, 25 and 50 mg/kg, i.p.) and carrageenan-induced leucocyte migration (1 and 5 mg/kg, i.p.) to mice peritoneal cavities. Besides, they also significantly inhibited MPO and LDH releases at doses ranging from 0.001 to 1 μg/ml. Thus, in general, the anti-inflammatory activity of Dox was higher as compared to that of Mino, although the radical scavenging activity of Mino was of a magnitude 10 times higher.

Conclusions

Our data indicate that anti-inflammatory and antioxidant effects, involve the inhibition of iNOS and TNF-alpha, among other properties, and these encourage clinical studies of these compounds for new therapeutic applications, especially those were inflammation plays a role.

Keywords

Minocycline Doxycycline Inflammation Cytokines Antioxidant effect