Skip to main content

Advertisement

Log in

Anti-Inflammatory Effects of Schisandrin B on LPS-Stimulated BV2 Microglia via Activating PPAR-γ

  • ORIGINAL ARTICLE
  • Published:
Inflammation Aims and scope Submit manuscript

Abstract

Schisandrin B (Sch B), a dibenzocyclooctadiene lignan isolated from Schisandra chinensis (Turcz.) Baill, has been shown to have anti-inflammatory effect. The purpose of this study was to evaluate the effect of Sch B on LPS-induced inflammation in microglia and to investigate the molecular targets of Sch B. BV2 cells were stimulated by LPS in the presence or absence of Sch B. The results showed that the levels of TNF-α, IL-6, IL-1β, and PGE2 upregulated by LPS were significantly suppressed by Sch B. LPS-induced NF-κB activation was also inhibited by Sch B. Furthermore, Sch B was found to upregulate the expression of PPAR-γ in a concentration-dependent manner. In addition, the inhibition of Sch B on TNF-α, IL-6, IL-1β, and PGE2 production were reversed by PPAR-γ antagonist GW9662. In conclusion, these results suggested that Sch B inhibited LPS-induced inflammatory response by activating PPAR-γ.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  1. Nimmerjahn, A., F. Kirchhoff, and F. Helmchen. 2005. Resting microglial cells are highly dynamic surveillants of brain parenchyma in vivo. Science 308: 1314–1318.

    Article  CAS  PubMed  Google Scholar 

  2. Ransohoff, R.M., and V.H. Perry. 2009. Microglial physiology: unique stimuli, specialized responses. Annual Review of Immunology 27: 119–145.

    Article  CAS  PubMed  Google Scholar 

  3. Kang, S.Y., H.W. Jung, M.Y. Lee, H.W. Lee, S.W. Chae, and Y.K. Park. 2014. Effect of the semen extract of Cuscuta chinensis on inflammatory responses in LPS-stimulated BV-2 microglia. Chinese Journal of Natural Medicines 12: 573–581.

    Article  CAS  PubMed  Google Scholar 

  4. Glass, C.K., K. Saijo, B. Winner, M.C. Marchetto, and F.H. Gage. 2010. Mechanisms underlying inflammation in neurodegeneration. Cell 140: 918–934.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Teeling, J.L., and V.H. Perry. 2009. Systemic infection and inflammation in acute CNS injury and chronic neurodegeneration: underlying mechanisms. Neuroscience 158: 1062–1073.

    Article  CAS  PubMed  Google Scholar 

  6. Hebert, L.E., L.A. Beckett, P.A. Scherr, and D.A. Evans. 2001. Annual incidence of Alzheimer disease in the United States projected to the years 2000 through 2050. Alzheimer Disease and Associated Disorders 15: 169–173.

    Article  CAS  PubMed  Google Scholar 

  7. Qian, L., P.M. Flood, and J.S. Hong. 2010. Neuroinflammation is a key player in Parkinson’s disease and a prime target for therapy. Journal of Neural Transmission 117: 971–979.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Minghetti, L. 2005. Role of inflammation in neurodegenerative diseases. Current Opinion in Neurology 18: 315–321.

    Article  CAS  PubMed  Google Scholar 

  9. Zeng, K.W., T. Zhang, H. Fu, G.X. Liu, and X.M. Wang. 2012. Schisandrin B exerts anti-neuroinflammatory activity by inhibiting the Toll-like receptor 4-dependent MyD88/IKK/NF-kappa B signaling pathway in lipopolysaccharide-induced microglia. European Journal of Pharmacology 692: 29–37.

    Article  CAS  PubMed  Google Scholar 

  10. Leong, P.K., and K.M. Ko. 2015. Schisandrin B induces an Nrf2-mediated thioredoxin expression and suppresses the activation of inflammasome in vitro and in vivo. BioFactors 41: 314–323.

    Article  CAS  PubMed  Google Scholar 

  11. Cai, Z., J. Liu, H. Bian, J. Cai, and G. Zhu. 2016. Suppression of P2X7/NF-kappaB pathways by Schisandrin B contributes to attenuation of lipopolysaccharide-induced inflammatory responses in acute lung injury. Archives of Pharmacal Research 39: 499–507.

    Article  CAS  PubMed  Google Scholar 

  12. Liu, W., Y. Liu, Z. Wang, T. Yu, Q. Lu, and H. Chen. 2015. Suppression of MAPK and NF-kappa B pathways by schisandrin B contributes to attenuation of DSS-induced mice model of inflammatory bowel disease. Pharmazie 70: 598–603.

    PubMed  Google Scholar 

  13. Block, M.L., L. Zecca, and J.S. Hong. 2007. Microglia-mediated neurotoxicity: uncovering the molecular mechanisms. Nature Reviews Neuroscience 8: 57–69.

    Article  CAS  PubMed  Google Scholar 

  14. Lull, M.E., and M.L. Block. 2010. Microglial activation and chronic neurodegeneration. Neurotherapeutics 7: 354–365.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  15. Long-Smith, C.M., A.M. Sullivan, and Y.M. Nolan. 2009. The influence of microglia on the pathogenesis of Parkinson’s disease. Progress in Neurobiology 89: 277–287.

    Article  CAS  PubMed  Google Scholar 

  16. Tweedie, D., K. Sambamurti, and N.H. Greig. 2007. TNF-alpha inhibition as a treatment strategy for neurodegenerative disorders: New drug candidates and targets. Current Alzheimer Research 4: 378–385.

    Article  CAS  PubMed  Google Scholar 

  17. Karin, M., and F.R. Greten. 2005. NF kappa B: Linking inflammation and immunity to cancer development and progression. Nature Reviews Immunology 5: 749–759.

    Article  CAS  PubMed  Google Scholar 

  18. Wang, H.Y., H. Wang, J.H. Wang, Q. Wang, Q.F. Ma, and Y.Y. Chen. 2015a. Protocatechuic Acid Inhibits Inflammatory Responses in LPS-Stimulated BV2 Microglia via NF-kappa B and MAPKs Signaling Pathways. Neurochemical Research 40: 1655–1660.

    Article  CAS  PubMed  Google Scholar 

  19. Chow, Y.L., K.H. Lee, S. Vidyadaran, N.H. Lajis, M.N. Akhtar, D.A. Israf, and A. Syahida. 2012. Cardamonin from Alpinia rafflesiana inhibits inflammatory responses in IFN-gamma/LPS-stimulated BV2 microglia via NF-kappa B signalling pathway. International Immunopharmacology 12: 657–665.

    Article  CAS  PubMed  Google Scholar 

  20. Zhu, J.Y., L. Jiang, Y.Q. Liu, W.Y. Qian, J.L. Liu, J. Zhou, R. Gao, H. Xiao, and J. Wang. 2015. MAPK and NF-kappa B Pathways Are Involved in Bisphenol A-Induced TNF-alpha and IL-6 Production in BV2 Microglial Cells. Inflammation 38: 637–648.

    Article  CAS  PubMed  Google Scholar 

  21. Wang, Y.M., J. Zhou, S. Fu, C. Wang, and B. Zhou. 2015b. Preventive Effects of Protocatechuic Acid on LPS-Induced Inflammatory Response in Human Gingival Fibroblasts via Activating PPAR-gamma. Inflammation 38: 1080–1084.

    Article  CAS  PubMed  Google Scholar 

  22. Takagi, T., Y. Naito, N. Tomatsuri, O. Handa, H. Ichikawa, N. Yoshida, and T. Yoshikawa. 2002. Pioglitazone, a PPAR-gamma ligand, provides protection from dextran sulfate sodium-induced colitis in mice in association with inhibition of the NF-kappa B-cytokine cascade. Redox Report 7: 283–289.

    Article  CAS  PubMed  Google Scholar 

  23. Zhang, Y.F., X.L. Zou, J. Wu, X.Q. Yu, and X. Yang. 2015. Rosiglitazone, a Peroxisome Proliferator-Activated Receptor (PPAR)-gamma Agonist, Attenuates Inflammation Via NF-kappa B Inhibition in Lipopolysaccharide-Induced Peritonitis. Inflammation 38: 2105–2115.

    Article  CAS  PubMed  Google Scholar 

  24. Corsi, L., B.M. Dongmo, and R. Avallone. 2015. Supplementation of omega 3 fatty acids improves oxidative stress in activated BV2 microglial cell line. International Journal of Food Sciences and Nutrition 66: 293–299.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Jin-Xu Zheng.

Ethics declarations

Conflict of Interest

All authors declare that they have no conflict of interest.

This work was supported by the grant guiding project for social development of Zhenjiang city (FZ2015036).

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Liu, N., Zheng, JX., Zhuang, YS. et al. Anti-Inflammatory Effects of Schisandrin B on LPS-Stimulated BV2 Microglia via Activating PPAR-γ. Inflammation 40, 1006–1011 (2017). https://doi.org/10.1007/s10753-017-0544-2

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10753-017-0544-2

KEY WORDS

Navigation