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miRNA-451a Targets IFN Regulatory Factor 8 for the Progression of Systemic Lupus Erythematosus

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Abstract

Increasing evidence has shown that miRNA-451a (miR-451a) is associated with the development of systemic lupus erythematosus (SLE); however, the mechanism of this association is not fully known. The present study found an increased expression of miR-451a in the spleen and thymus of an SLE mice model. A decrease in miR-451a expression partly relieved the enlargement of the spleen and decreased the proteinuria content and immune complex deposits. The deficiency in miR-451a also decreased numbers of CD4+CD69+ and CD4+/CD8+ T cells and the levels of the serum cytokines IL-17a and IL-4. The IFN regulatory factor (IRF) 8 was highly expressed in the immune organs of wild-type mice but was suppressed in SLE-like mice. A dual-luciferase reporter assay was carried out in combination with gene silencing and overexpression to verify that IRF8 was a target of miR-451a in vitro and in vivo. The data indicate the function and a target of miR-451a in SLE, providing a new target for SLE therapy.

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Abbreviations

CBB:

Coomassie brilliant blue

ELISA:

Enzyme-linked immunosorbent assay

IRF8:

IFN regulatory factor 8

miR-451a:

miRNA-451a

SLE:

Systemic lupus erythematosus

Tfh:

T-follicular helper

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Correspondence to Rui Wu.

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Cheng, J., Wu, R., Long, L. et al. miRNA-451a Targets IFN Regulatory Factor 8 for the Progression of Systemic Lupus Erythematosus. Inflammation 40, 676–687 (2017). https://doi.org/10.1007/s10753-017-0514-8

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