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Therapeutic Effects of Resveratrol in a Mouse Model of LPS and Cigarette Smoke-Induced COPD

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Abstract

This study was designed to examine whether resveratrol exerts the protective effects on LPS and cigarette smoke (LC)-induced COPD in a murine model. In lung histopathological studies, H&E, Masson’s trichrome, and AB-PAS staining were performed. The cytokines (IL-6, IL-17, TGF-β, and TNF-α) and inflammatory cells in BALF were determined. The Beclin1 level in the lungs of mouse was analyzed. Compared with the LC-induced mouse, the level of inflammatory cytokines (IL-17, IL-6, TNF-α, and TGF-β) of the BALF in the resveratrol + cigarette smoke-treated mouse had obviously decreased. Histological examination of the lung tissue revealed that the resveratrol treatment attenuated the fibrotic response and mucus hypersecretion. In addition, resveratrol inhibited the expression of the Beclin1 protein in mouse lungs. The presented findings collectively suggest that resveratrol has a therapeutic effect on mouse LC-induced COPD, and its mechanism of action might be related to reducing the production of the Beclin1 protein.

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Abbreviations

COPD:

Chronic obstructive pulmonary disease

DEX:

Dexamethasone

BALF:

Bronchoalveolar lavage fluid

PBS:

Phosphate buffered physiological saline

RES:

Resveratrol

LC:

LPS + cigarette smoke

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Acknowledgments

This work was supported by the Jiangxi Province Education Office Youth Scientific Research Fund (No.GJJ14226) and Jiangxi Province Health and Family Planning Commission Fund (2014A070).

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Authors and Affiliations

  1. School of Resources, Environmental & Chemical Engineering of Nanchang University, Nanchang, 330031, China

    Jinlong Chen, Weiya Zhang, Danhua Peng, Yanan Xia, Yi Lu, Jing Zhu & Renping Liu

  2. Medical Experiment Education Department, Medical College of Nanchang University, Nanchang, 330031, China

    Xu Yang

  3. Department of Anesthesia, Wenzhou Central Hospital, Wenzhou, 325000, China

    Xiaodong Han

  4. Department of Neurology, Xiangyang Central Hospital, Xiangyang, 441000, China

    Guangjie Song

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  1. Jinlong Chen
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  2. Xu Yang
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Correspondence to Renping Liu.

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Chen, J., Yang, X., Zhang, W. et al. Therapeutic Effects of Resveratrol in a Mouse Model of LPS and Cigarette Smoke-Induced COPD. Inflammation 39, 1949–1959 (2016). https://doi.org/10.1007/s10753-016-0430-3

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  • DOI: https://doi.org/10.1007/s10753-016-0430-3

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