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The Effects of IL-22 on the Inflammatory Mediator Production, Proliferation, and Barrier Function of HUVECs

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Abstract

The aim of this study was to investigate the effects of interleukin (IL)-22 on proliferation function and inflammatory mediator production and barrier function of human umbilical vein endothelial cells (HUVECs). The expression of mRNA was detected by RT-PCR. The proliferation ability of cells was evaluated using a cell counting kit assay. Real-time quantitative PCR and Western blot were used to detect the expression of inflammatory mediators. The endothelial barrier permeability was assessed by measuring permeability to FITC-labeled dextran. The distribution of occludin was detected by immunofluorescence. IL-22R1 mRNA expression was noted in HUVECs. IL-22 could enhance the proliferation ability of HUVECs and suppress lipopolysaccharide (LPS)-induced proliferation inhibition in these cells. IL-22 also enhanced the production of CCL2 and CCL20 by HUVECs. Besides, IL-22 could improve barrier function and decrease LPS-induced increased cellular permeability and inhibit the LPS-induced destruction of occludin in HUVECs. IL-22 may play a protective role in the development of vasculitis.

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Authors and Affiliations

  1. The First Affiliated Hospital of Chongqing Medical University, Chongqing, People’s Republic of China

    Xian He, Hui Li, Ying Chen, Aijun Chen, Kui Shan, Jin Chen, Hengguang Zhao, Xiaojiao Zhang & Tao Cai

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  1. Xian He
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  2. Hui Li
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  3. Ying Chen
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  4. Aijun Chen
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  6. Jin Chen
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  7. Hengguang Zhao
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  8. Xiaojiao Zhang
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  9. Tao Cai
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Correspondence to Tao Cai.

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He, X., Li, H., Chen, Y. et al. The Effects of IL-22 on the Inflammatory Mediator Production, Proliferation, and Barrier Function of HUVECs. Inflammation 39, 1099–1107 (2016). https://doi.org/10.1007/s10753-016-0341-3

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  • DOI: https://doi.org/10.1007/s10753-016-0341-3

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