Skip to main content
SpringerLink
Log in

Inhibition of Radiation-Induced Oxidative Damage in the Lung Tissue: May Acetylsalicylic Acid Have a Positive Role?

  • Published:
Inflammation Aims and scope Submit manuscript

Abstract

The lung is relatively sensitive to irradiation. It is shown that acetylsalicylic acid (ASA) might reduce oxidative injury and that it has a place in protection from cancer. The aim of this study is to evaluate the potential radioprotective effects of ASA. Whole-body irradiation (6 Gy, single dose) was applied to the rats. Glutathione (GSH), malondialdehyde (MDA), myeloperoxidase (MPO), and nitric oxide (NO) levels in the lung tissue were measured. Control (C), Radiation (R), Radiation + ASA (R + ASA; received irradiation and 25 mg/kg of ASA intraperitoneally (i.p.)), and Radiation + Amifostine (R + WR-2721; received irradiation and 200 mg/kg of WR-2721 i.p.) groups were used. The MPO levels decreased statistically significantly in the group administered ASA. Histopathologically, a radioprotective effect of ASA was more evident in the R + ASA group. ASA is an agent which has not been used as a radioprotector in the clinic yet, and it is worth supporting with more advanced studies.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

References

  1. Marks, L.B., X. Yu, Z. Vujaskovic, et al. 2003. Radiation-induced lung injury. Seminars in Radiation Oncology 13: 333–345.

    Article  PubMed  Google Scholar 

  2. Hill, R.P. 2005. Radiation effects on the respiratory system. The British Institute of Radiology 27: 75–81.

    Article  Google Scholar 

  3. Yarnold, J., and M.C. Brotons. 2010. Pathogenetic mechanisms in radiation fibrosis. Radiotherapy Oncology 97: 149–161.

    Article  CAS  PubMed  Google Scholar 

  4. Davis, S.D., D.F. Yankelevitz, and C.I. Henschke. 1992. Radiation effects on the lung: clinical features, pathology, and imaging findings. American Journal of Roentgenology 159: 1157–1164.

    Article  CAS  PubMed  Google Scholar 

  5. Wynn, T.A. 2011. Integrating mechanisms of pulmonary fibrosis. Journal of Experimental Medicine 208: 1339–1350.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  6. Weiss, J.F. 1997. Pharmacologic approaches to protection against radiation-induced lethality and other damage. Environmental Health Perspectives 105: 1473–1478.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  7. Linard, C., O. Grémy, and M. Benderitter. 2008. Reduction of peroxisome proliferation-activated receptor gamma expression by gamma-irradiation as a mechanism contributing to inflammatory response in rat colon: modulation by the 5-aminosalicylic acid agonist. Journal of Pharmacology and Experimental Therapeutics 324: 911–920.

    Article  CAS  PubMed  Google Scholar 

  8. Demirel, C., S. Kilçiksiz, O.I. Ay, S. Gürgül, M.N. Ay, and N. Erdal. 2009. Effect of N-acetylcysteine on radiation-induced genotoxicity and cytotoxicity in rat bone marrow. Journal of Radiation Research 50: 43–50.

    Article  CAS  PubMed  Google Scholar 

  9. Warner, T.D., and J.A. Mitchell. 2002. Cyclooxygenase-3 (COX-3): filling in the gaps toward a COX continuum? Proceeding of the Nationall Academy of Sciences U S A 99: 13371–13373.

    Article  CAS  Google Scholar 

  10. Mantena, S.K., and M.K. Chandrasekharan. 2008. Radioprotection by copper and zinc complexes of 5-aminosalicylic acid: a preliminary study. Journal of Environmental Pathology, Toxicology and Oncology 27: 123–134.

    Article  CAS  PubMed  Google Scholar 

  11. Chan, A.T., E.L. Giovannucci, J.A. Meyerhardt, E.S. Schernhammer, G.C. Curhan, and C.S. Fuchs. 2005. Long-term use of aspirin and nonsteroidal anti-inflammatory drugs and risk of colorectal cancer. JAMA 294: 914–923.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  12. Moysich, K.B., R.J. Menezes, A. Ronsani, et al. 2002. Regular aspirin use and lung cancer risk. BMC Cancer 2: 31.

    Article  PubMed Central  PubMed  Google Scholar 

  13. Kilciksiz, S., C. Demirel, N. Erdal, S. Gürgül, L. Tamer, L. Ayaz, and Y. Ors. 2008. The effect of N-acetylcysteine on biomarkers for radiation-induced oxidative damage in a rat model. Acta Medica Okayama 62: 403–409.

    CAS  PubMed  Google Scholar 

  14. Capton, G.D., J.C. Cook, and M.E. Hurtt. 2003. Relationship between cyclooxygenase 1 and 2 selective inhibitors and fetal development when administered to rats and rabbits during the sensitive periods for heart development and midline closure. Birth Defects Research Part B Reproductive Toxicology 68: 47–56.

    Article  Google Scholar 

  15. Beutler, E., and T. Gelbart. 1986. Improved assay of the enzymes of glutathione synthesis: gamma-glutamylcysteine synthetase and glutathione synthetase. Clinica Chimica Acta 158: 115–123.

    Article  CAS  Google Scholar 

  16. Lowry, O.H., N.J. Rosebrough, A.L. Farr, and R.J. Randall. 1951. Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193: 265–275.

    CAS  PubMed  Google Scholar 

  17. Yagi, K. 1994. Lipid peroxides and related radicals in clinical medicine. In: Free radicals in diagnostic medicine, ed. Armstrong, D. p. 1–15 New York, Plenum

  18. Golowich, S.P. 1955. Methods in enzymology, vol. IIth ed, 769. New York: Academic Press.

    Google Scholar 

  19. Molteni, A., J.E. Moulder, E.F. Cohen, et al. 2000. Control of radiation-induced pneumopathy and lung fibrosis by angiotensin-converting enzyme inhibitors and an angiotensin II type 1 receptor blocker. International Journal of Radiation Biology 76: 523–532.

    Article  CAS  PubMed  Google Scholar 

  20. Hong, J.H., C.S. Chiang, C.Y. Tsao, P.Y. Lin, C.J. Wu, and W.H. McBride. 2001. Can short-term administration of dexamethasone abrogate radiation-induced acute cytokine gene response in lung and modify subsequent molecular responses? International Journal of Radiation Oncology Biology Physics 51: 296–303.

    Article  CAS  Google Scholar 

  21. Vujaskovic, Z., Q.F. Feng, Z.N. Rabbani, M.S. Anscher, T.V. Samulski, and D.M. Brizel. 2002. Radioprotection of lungs by amifostine is associated with reduction in profibrogenic cytokine activity. Radiation Research 157: 656–660.

    Article  CAS  PubMed  Google Scholar 

  22. Shi, X., M. Ding, Z. Dong, et al. 1999. Antioxidant properties of aspirin: characterization of the ability of aspirin to inhibit silica-induced lipid peroxidation, DNA damage, NF-kappaB activation, and TNF-alpha production. Molecular and Cellular Biochemistry 199: 93–102.

    Article  CAS  PubMed  Google Scholar 

  23. Asanuma, M., S. Nishibayashi-Asanuma, I. Miyazaki, M. Kohno, and N. Ogawa. 2001. Neuroprotective effects of non-steroidal anti-inflammatory drugs by direct scavenging of nitric oxide radicals. Journal of Neurochemistry 76: 1895–1904.

    Article  CAS  PubMed  Google Scholar 

  24. Verheij, M., F.A. Stewart, Y. Oussoren, J.J. Weening, and L. Dewit. 1995. Amelioration of radiation nephropathy by acetylsalicylic acid. International Journal of Radiation Biology 67: 587–596.

    Article  CAS  PubMed  Google Scholar 

  25. Drew, J.E., J.R. Arthur, A.J. Farquharson, W.R. Russell, P.C. Morrice, and G.G. Duthie. 2005. Salicylic acid modulates oxidative stress and glutathione peroxidase activity in the rat colon. Biochemical Pharmacology 70: 888–893.

    Article  CAS  PubMed  Google Scholar 

  26. Cantin, A.M., S.L. North, G.A. Fells, R.C. Hubbard, and R.G. Crystal. 1987. Oxidant-mediated epithelial cell injury in idiopathic pulmonary fibrosis Journal Of Clinical Investigation 79: 1665–1673.

    Google Scholar 

  27. Hoggatt, J., P. Singh, K.N. Stilger, P.A. Pelt, et al. 2013. Recovery from hematopoietic injury by modulating prostaglandin E(2) signaling post-irradiation. Blood Cells Molecules Diseases 50: 147–153.

    Article  CAS  Google Scholar 

  28. Van Kleef, E.M., J.A. Te Poele, Y.G. Oussoren, A. Van der Wal, L.G. Dewit, and F.A. Stewart. 2000. Influence of acetylsalicylic acid on development of radiation-induced nephropathy. International Journal of Radiation Biology 76: 1565–1573.

    Article  PubMed  Google Scholar 

  29. Mohamed D.G., and R. M. Amin, 2015. Involvement of heat shock proteins 60 in acetylsalicylic acid radioprotection of Albino rat submandibular salivary gland Journal of Radiation Research and Applied Sciences (article in press).

  30. Khoshnan, A., J. Ko, S. Tescu, P. Brundin, and P.H. Patterson. 2009. IKKalpha and IKKbeta regulation of DNA damage-induced cleavage of huntingtin. PloS One 4: e57–68.

    Article  Google Scholar 

  31. Huang, C., W.Y. Ma, D. Hanenberger, M.P. Cleary, G.T. Bowden, and Z. Dong. 1997. Inhibition of ultraviolet B-induced activator protein-1 (AP-1) activity by aspirin in AP-1-luciferase transgenic mice. Journal of Biological Chemistry 272: 26325–26331.

    Article  CAS  PubMed  Google Scholar 

  32. Dong, Z., C. Huang, R.E. Brown, and W.Y. Ma. 1997. Inhibition of activator protein 1 activity and neoplastic transformation by aspirin. Journal Of Biological Chemistry 272: 9962–9970.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  33. Bair, W.B., N. Hart, J. Einspahr, et al. 2002. Inhibitory effects of sodium salicylate and acetylsalicylic acid on UVB-induced mouse skin carcinogenesis. Cancer Epidemiol Biomarkers Prevention 11: 1645–1652.

    CAS  Google Scholar 

  34. Awtry, E.H., and J. Loscalzo. 2000. Aspirin. Circulation 101: 1206–1218.

    Article  CAS  PubMed  Google Scholar 

  35. Weitz, J., M. Koch, J. Debus, T. Hohler, P.R. Galle, and M.W. Buchler. 2005. Colorectal cancer. Lancet 365: 153–165.

    Article  PubMed  Google Scholar 

  36. Niho, N., T. Kitamura, M. Takahashi, et al. 2006. Suppression ofazoxymethane-induced colon cancer development in rats by a cyclooxygenase-1 selective inhibitor, mofezolac. Cancer Science 97: 1011–1014.

    Article  CAS  PubMed  Google Scholar 

  37. Mennie, A.T., V.M. Dalley, L.C. Dinneen, and H.O. Collier. 1975. Treatment of radiation-induced gastrointestinal distress with acetylsalicylate. Lancet 2: 942–943.

    Article  CAS  PubMed  Google Scholar 

Download references

Conflict of interest

The authors declare that there is no conflict of interest.

Author information

Authors and Affiliations

  1. Department of Biophysics, Faculty of Medicine, Gaziantep University, 27310, Gaziantep, Turkey

    Can Demirel

  2. Department of Radiation Oncology, Kolan International Hospital, 34000, Istanbul, Turkey

    Sevil Cagiran Kilciksiz

  3. Department of Biophysics, Faculty of Medicine, Gaziosmanpaşa University, 60000, Tokat, Turkey

    Serkan Gurgul

  4. Department of Biophysics, Faculty of Medicine, Mersin University, 33169, Mersin, Turkey

    Nurten Erdal

  5. Department of Pathology, Izmir Atatürk Training and Research Hospital, 35000, Izmir, Turkey

    Seyran Yigit

  6. Department of Biochemistry, Faculty of Medicine, Mersin University, 33169, Mersin, Turkey

    Lulufer Tamer

  7. Department of Biochemistry, Faculty of Pharmacy, University of Trakya, 22000, Edirne, Turkey

    Lokman Ayaz

Authors
  1. Can Demirel
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Sevil Cagiran Kilciksiz
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Serkan Gurgul
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Nurten Erdal
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Seyran Yigit
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Lulufer Tamer
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Lokman Ayaz
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Can Demirel.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Demirel, C., Kilciksiz, S.C., Gurgul, S. et al. Inhibition of Radiation-Induced Oxidative Damage in the Lung Tissue: May Acetylsalicylic Acid Have a Positive Role?. Inflammation 39, 158–165 (2016). https://doi.org/10.1007/s10753-015-0234-x

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10753-015-0234-x

KEY WORDS

Search

Navigation