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Punicalagin Ameliorates Lipopolysaccharide-Induced Acute Respiratory Distress Syndrome in Mice

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Abstract

Punicalagin, a bioactive ellagitannin isolated from pomegranate, has been reported to have anti-inflammatory property. In the present study, we analyzed the role of punicalagin against acute respiratory distress syndrome (ARDS) induced by lipopolysaccharide (LPS) in mice. Male BALB/c mice with ARDS, induced by intranasal instillation of LPS, were treated with punicalagin 1 h prior to LPS exposure. The effects of punicalagin on pro-inflammatory cytokines, myeloperoxidase activity, nuclear factor kappa B (NF-κB) activation, and the histopathological changes were evaluated. The results showed that punicalagin treatment attenuated LPS-induced lung edema, elevating TNF-α, IL-6, and IL-1β levels in the bronchoalveolar lavage fluid (BALF). Meanwhile, punicalagin significantly inhibited LPS-induced increases in the macrophage and neutrophil infiltration of lung tissues and myeloperoxidase activity. Furthermore, punicalagin inhibits Toll-like receptor 4 (TLR4) expression and NF-κB activation induced by LPS. In conclusion, this is the first study to demonstrate that punicalagin protects against LPS-induced ARDS in mice. The underlying mechanisms may include inhibition of TLR4-mediated NF-κB signaling pathways.

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References

  1. Baumann, W.R., R.C. Jung, M. Koss, C.T. Boylen, L. Navarro, and O.P. Sharma. 1986. Incidence and mortality of adult respiratory distress syndrome: a prospective analysis from a large metropolitan hospital. Critical Care Medicine 14: 1–4.

    Article  CAS  PubMed  Google Scholar 

  2. Ware, L.B. 2006. Pathophysiology of acute lung injury and the acute respiratory distress syndrome. Seminars in Respiratory and Critical Care Medicine 27: 337–349.

    Article  PubMed  Google Scholar 

  3. Tomashefski Jr., J.F. 2000. Pulmonary pathology of acute respiratory distress syndrome. Clinics in Chest Medicine 21: 435–466.

    Article  PubMed  Google Scholar 

  4. Mei, S.H., S.D. McCarter, Y. Deng, C.H. Parker, W.C. Liles, and D.J. Stewart. 2007. Prevention of LPS-induced acute lung injury in mice by mesenchymal stem cells overexpressing angiopoietin 1. PLoS Medicine 4: e269.

    Article  PubMed Central  PubMed  Google Scholar 

  5. Imai, Y., K. Kuba, G.G. Neely, R. Yaghubian-Malhami, T. Perkmann, G. van Loo, M. Ermolaeva, R. Veldhuizen, Y.H. Leung, H. Wang, et al. 2008. Identification of oxidative stress and Toll-like receptor 4 signaling as a key pathway of acute lung injury. Cell 133: 235–249.

    Article  CAS  PubMed  Google Scholar 

  6. Gunnarsson, P.S., K. Sigvaldason, K.I. Reynisson, and A.D. Moller. 2013. The incidence and mortality of ARDS at Landspitali—The National University Hospital of Iceland 2004–2008. Læknablađiđ 99: 443–448.

    PubMed  Google Scholar 

  7. Seeram, N.P., L.S. Adams, S.M. Henning, Y. Niu, Y. Zhang, M.G. Nair, and D. Heber. 2005. In vitro antiproliferative, apoptotic and antioxidant activities of punicalagin, ellagic acid and a total pomegranate tannin extract are enhanced in combination with other polyphenols as found in pomegranate juice. Journal of Nutritional Biochemistry 16: 360–367.

    Article  CAS  PubMed  Google Scholar 

  8. Cerda, B., R. Llorach, J.J. Ceron, J.C. Espin, and F.A. Tomas-Barberan. 2003. Evaluation of the bioavailability and metabolism in the rat of punicalagin, an antioxidant polyphenol from pomegranate juice. European Journal of Nutrition 42: 18–28.

    Article  CAS  PubMed  Google Scholar 

  9. Lin, C.C., Y.F. Hsu, and T.C. Lin. 1999. Effects of punicalagin and punicalin on carrageenan-induced inflammation in rats. American Journal of Chinese Medicine 27: 371–376.

    Article  CAS  PubMed  Google Scholar 

  10. Xu, X, Yin, P, Wan, C, Chong, X, Liu, M, Cheng, P, Chen, J, Liu, F, Xu, J. 2014. Punicalagin inhibits inflammation in LPS-induced RAW264.7 macrophages via the suppression of TLR4-mediated MAPKs and NF-kappaB activation. Inflammation

  11. Lee, C., L. Chen, and C. Wang. 2010. Anti-inflammatory effects of Punica granatum Linne in LPS-induced primary human chondrocytes. Planta Medica 76: 1376–1376.

    Google Scholar 

  12. Jean-Gilles, D., L. Li, V.G. Vaidyanathan, R. King, B. Cho, D.R. Worthen, C.O. Chichester 3rd, and N.P. Seeram. 2013. Inhibitory effects of polyphenol punicalagin on type-II collagen degradation in vitro and inflammation in vivo. Chemico-Biological Interactions 205: 90–99.

    Article  CAS  PubMed  Google Scholar 

  13. Lee, W.L., and G.P. Downey. 2001. Neutrophil activation and acute lung injury. Current Opinion in Critical Care 7: 1–7.

    Article  CAS  PubMed  Google Scholar 

  14. Zmijewski, J.W., E. Lorne, X. Zhao, Y. Tsuruta, Y. Sha, G. Liu, and E. Abraham. 2009. Antiinflammatory effects of hydrogen peroxide in neutrophil activation and acute lung injury. American Journal of Respiratory and Critical Care Medicine 179: 694–704.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  15. Graff, G., D.A. Gamache, M.T. Brady, J.M. Spellman, and J.M. Yanni. 1998. Improved myeloperoxidase assay for quantitation of neutrophil influx in a rat model of endotoxin-induced uveitis. Journal of Pharmacological and Toxicological Methods 39: 169–178.

    Article  CAS  PubMed  Google Scholar 

  16. Goodman, R.B., J. Pugin, J.S. Lee, and M.A. Matthay. 2003. Cytokine-mediated inflammation in acute lung injury. Cytokine & Growth Factor Reviews 14: 523–535.

    Article  CAS  Google Scholar 

  17. Giebelen, I.A.J., D.J. van Westerloo, G.J. LaRosa, A.F. de Vos, and T. van der Poll. 2007. Local stimulation of alpha 7 cholinergic receptors inhibits LPS-induced TNF-alpha release in the mouse lung. Shock 28: 700–703.

    CAS  PubMed  Google Scholar 

  18. Kolb, M., P.J. Margetts, D.C. Anthony, F. Pitossi, and J. Gauldie. 2001. Transient expression of IL-1beta induces acute lung injury and chronic repair leading to pulmonary fibrosis. Journal of Clinical Investigation 107: 1529–1536.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  19. Parsons, P.E., M.D. Eisner, B.T. Thompson, M.A. Matthay, M. Ancukiewicz, G.R. Bernard, and A.P. Wheeler. 2005. Network NARDSCT: Lower tidal volume ventilation and plasma cytokine markers of inflammation in patients with acute lung injury. Critical Care Medicine 33: 1–6. discussion 230–232.

    Article  CAS  PubMed  Google Scholar 

  20. Lu, Y.C., W.C. Yeh, and P.S. Ohashi. 2008. LPS/TLR4 signal transduction pathway. Cytokine 42: 145–151.

    Article  CAS  PubMed  Google Scholar 

  21. Medzhitov, R., and J.C. Kagan. 2006. Phosphoinositide-mediated adaptor recruitment controls Toll-like receptor signaling. Cell 125: 943–955.

    Article  PubMed  Google Scholar 

  22. Gilmore, T.D. 2006. Introduction to NF-kappaB: players, pathways, perspectives. Oncogene 25: 6680–6684.

    Article  CAS  PubMed  Google Scholar 

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All authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Oncology, The General Hospital of Chengdu Military District, Chengdu, Sichuan, 610000, People’s Republic of China

    Jingjing Peng, Dong Wei, Zengqiang Fu, Dong Li, Yong Tan, Tao Xu, Jinjun Zhou & Tao Zhang

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  1. Jingjing Peng
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  2. Dong Wei
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  3. Zengqiang Fu
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  4. Dong Li
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  8. Tao Zhang
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Correspondence to Tao Zhang.

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Peng, J., Wei, D., Fu, Z. et al. Punicalagin Ameliorates Lipopolysaccharide-Induced Acute Respiratory Distress Syndrome in Mice. Inflammation 38, 493–499 (2015). https://doi.org/10.1007/s10753-014-9955-5

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