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Protective Effects of Lactobacillus plantarum NDC 75017 Against Lipopolysaccharide-Induced Liver Injury in Mice

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Abstract

This study investigated the protective effect of Lactobacillus plantarum NDC 75017 (L. plantarum NDC 75017) against acute liver injury induced by lipopolysaccharide (LPS). Thirty male mice were randomly divided into the control, LPS, and LPS + L. plantarum NDC 75017 groups. In the LPS + L. plantarum group, the mice were orally pretreated with L. plantarum NDC 75017 for 15 days. At 16 days, the mice in the LPS and LPS + L. plantarum NDC 75017 groups were intraperitoneally injected with LPS at 4 mg/kg body weight, whereas the control mice were treated with an equal amount of saline. After 8 h, the serum alanine transaminase (ALT), aspartate aminotransferase (AST), and histology changes were examined. The oxidative stress markers and pro-inflammatory cytokines in the liver were also examined. Meanwhile, the expression of nuclear factor κB (NF-κB) mRNA and toll-like receptor 4 (TLR4) in the liver was determined by qRT-PCR. The LPS group showed an increase in ALT and AST, whereas the LPS + L. plantarum NDC 75017 group showed a significant decrease. In addition, pretreatment with L. plantarum NDC 75017 can attenuate LPS-induced oxidative stress and inflammatory response. Furthermore, the increase of hepatic NF-κB and TLR4 mRNA induced by LPS was significantly downregulated by the pretreatment with L. plantarum NDC 75017. These data show that pretreatment with L. plantarum NDC 75017 protects against LPS-induced oxidative stress and inflammatory injury in the liver of mice, which may be attributed to the inhibition of the TLR4-NF-κB pathway.

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References

  1. Laubach, H.J., P.M. Jakob, K.O. Loevblad, A.E. Baird, M.P. Bovo, R.R. Edelman, and S. Warach. 1998. A phantom for diffusion-weighted imaging of acute stroke. Journal of Magnetic Resonance Imaging 8: 1349–1354.

    Google Scholar 

  2. Yang, R.B., M.R. Mark, A. Gray, A. Huang, M.H. Xie, M. Zhang, A. Goddard, W.I. Wood, A.L. Gurney, and P.J. Godowski. 1998. Toll-like receptor-2 mediates lipopolysaccharide-induced cellular signalling. Nature 395: 284–288.

    Article  PubMed  CAS  Google Scholar 

  3. Feng, H., W. Guo, J. Han, and X.A. Li. 2013. Role of caveolin-1 and caveolae signaling in endotoxemia and sepsis. Life Sciences 93: 1–6.

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  4. Winrow, V.R., P.G. Winyard, C.J. Morris, and D.R. Blake. 1993. Free radicals in inflammation: second messengers and mediators of tissue destruction. British Medical Bulletin 49: 506–522.

    PubMed  CAS  Google Scholar 

  5. Qureshi, S.T., P. Gros, and D. Malo. 1999. Host resistance to infection: genetic control of lipopolysaccharide responsiveness by TOLL-like receptor genes. Trends in Genetics 15: 291–294.

    Article  PubMed  CAS  Google Scholar 

  6. Shi, H., M.V. Kokoeva, K. Inouye, I. Tzameli, H. Yin, and J.S. Flier. 2006. TLR4 links innate immunity and fatty acid-induced insulin resistance. Journal of Clinical Investigation 116: 3015–3025.

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  7. Jaeschke, H. 2011. Reactive oxygen and mechanisms of inflammatory liver injury: present concepts. Journal of Gastroenterology and Hepatology 26(Suppl 1): 173–179.

    Article  PubMed  CAS  Google Scholar 

  8. Cohen, J. 2002. The immunopathogenesis of sepsis. Nature 420: 885–891.

    Article  PubMed  CAS  Google Scholar 

  9. Cross, M.L. 2002. Microbes versus microbes: immune signals generated by probiotic lactobacilli and their role in protection against microbial pathogens. FEMS Immunology and Medical Microbiology 34: 245–253.

    Article  PubMed  CAS  Google Scholar 

  10. Seimon, T.A., A. Obstfeld, K.J. Moore, D.T. Golenbock, and I. Tabas. 2006. Combinatorial pattern recognition receptor signaling alters the balance of life and death in macrophages. Proceedings of the National Academy of Sciences of the United States of America 103: 19794–19799.

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  11. Ben Ari, Z., O. Avlas, O. Pappo, V. Zilbermints, Y. Cheporko, L. Bachmetov, R. Zemel, A. Shainberg, E. Sharon, F. Grief, and E. Hochhauser. 2012. Reduced hepatic injury in Toll-like receptor 4-deficient mice following D-galactosamine/lipopolysaccharide-induced fulminant hepatic failure. Cellular Physiology and Biochemistry 29: 41–50.

    Article  PubMed  CAS  Google Scholar 

  12. Wang, Y., Y. Li, J. Xie, Y. Zhang, J. Wang, X. Sun, and H. Zhang. 2013. Protective effects of probiotic Lactobacillus casei Zhang against endotoxin- and d-galactosamine-induced liver injury in rats via anti-oxidative and anti-inflammatory capacities. International Immunopharmacology 15: 30–37.

    Article  PubMed  CAS  Google Scholar 

  13. Xu, R.Y., Y.P. Wan, Q.Y. Fang, W. Lu, and W. Cai. 2012. Supplementation with probiotics modifies gut flora and attenuates liver fat accumulation in rat nonalcoholic fatty liver disease model. Journal of Clinical Biochemistry and Nutrition 50: 72–77.

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  14. Wang, Y., Y. Liu, A. Sidhu, Z. Ma, C. McClain, and W. Feng. 2012. Lactobacillus rhamnosus GG culture supernatant ameliorates acute alcohol-induced intestinal permeability and liver injury. American Journal of Physiology - Gastrointestinal and Liver Physiology 303: G32–G41.

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  15. Ewaschuk, J., R. Endersby, D. Thiel, H. Diaz, J. Backer, M. Ma, T. Churchill, and K. Madsen. 2007. Probiotic bacteria prevent hepatic damage and maintain colonic barrier function in a mouse model of sepsis. Hepatology 46: 841–850.

    Article  PubMed  CAS  Google Scholar 

  16. Rishi, P., S.K. Mavi, S. Bharrhan, G. Shukla, and R. Tewari. 2009. Protective efficacy of probiotic alone or in conjunction with a prebiotic in Salmonella-induced liver damage. FEMS Microbiology Ecology 69: 222–230.

    Article  PubMed  CAS  Google Scholar 

  17. O'Sullivan, D.J. 2008. Genomics can advance the potential for probiotic cultures to improve liver and overall health. Current Pharmaceutical Design 14: 1376–1381.

    Article  PubMed  Google Scholar 

  18. Hathout, A.S., S.R. Mohamed, A.A. El-Nekeety, N.S. Hassan, S.E. Aly, and M.A. Abdel-Wahhab. 2011. Ability of Lactobacillus casei and Lactobacillus reuteri to protect against oxidative stress in rats fed aflatoxins-contaminated diet. Toxicon 58: 179–186.

    Article  PubMed  CAS  Google Scholar 

  19. Liu, Y., C. Man, X. Lv, L. Han, Y. Xue, X. Yang, Y. Shan, Y. Zhang, and Y. Jiang. 2012. Lactobacillus plantarum NDC 75017 affects il-6 gene expression in Caco-2 cells. Wei Sheng Wu Xue Bao 52: 1237–1243.

    PubMed  CAS  Google Scholar 

  20. Livak, K.J., and T.D. Schmittgen. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-delta delta C(T)) method. Methods 25: 402–408.

    Article  PubMed  CAS  Google Scholar 

  21. Hochhauser, E., O. Avlas, R. Fallach, L. Bachmetov, R. Zemel, O. Pappo, A. Shainberg, and Z. Ben Ari. 2013. Bone marrow and nonbone marrow Toll like receptor 4 regulate acute hepatic injury induced by endotoxemia. PLoS One 8: e73041.

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  22. Rishi, P., S. Bharrhan, G. Singh, and I.P. Kaur. 2011. Effect of Lactobacillus plantarum and L-arginine against endotoxin-induced liver injury in a rat model. Life Sciences 89: 847–853.

    Article  PubMed  CAS  Google Scholar 

  23. Nardone, G., D. Compare, E. Liguori, V. Di Mauro, A. Rocco, M. Barone, A. Napoli, D. Lapi, M.R. Iovene, and A. Colantuoni. 2010. Protective effects of Lactobacillus paracasei F19 in a rat model of oxidative and metabolic hepatic injury. American Journal of Physiology - Gastrointestinal and Liver Physiology 299: G669–G676.

    Article  PubMed  CAS  Google Scholar 

  24. Kaur, G., N. Tirkey, S. Bharrhan, V. Chanana, P. Rishi, and K. Chopra. 2006. Inhibition of oxidative stress and cytokine activity by curcumin in amelioration of endotoxin-induced experimental hepatoxicity in rodents. Clinical and Experimental Immunology 145: 313–321.

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  25. Moncada, S., R.M. Palmer, and E.A. Higgs. 1991. Nitric oxide: physiology, pathophysiology, and pharmacology. Pharmacological Reviews 43: 109–142.

    PubMed  CAS  Google Scholar 

  26. Brashears, M.M., D. Jaroni, and J. Trimble. 2003. Isolation, selection, and characterization of lactic acid bacteria for a competitive exclusion product to reduce shedding of Escherichia coli O157:H7 in cattle. Journal of Food Protection 66: 355–363.

    PubMed  CAS  Google Scholar 

  27. Hamilton-Miller, J.M. 2003. The role of probiotics in the treatment and prevention of Helicobacter pylori infection. International Journal of Antimicrobial Agents 22: 360–366.

    Article  PubMed  CAS  Google Scholar 

  28. Sartor, R.B. 2005. Probiotic therapy of intestinal inflammation and infections. Current Opinion in Gastroenterology 21: 44–50.

    PubMed  Google Scholar 

  29. Deguchi, R., H. Nakaminami, E. Rimbara, N. Noguchi, M. Sasatsu, T. Suzuki, M. Matsushima, J. Koike, M. Igarashi, H. Ozawa, R. Fukuda, and A. Takagi. 2012. Effect of pretreatment with Lactobacillus gasseri OLL2716 on first-line Helicobacter pylori eradication therapy. Journal of Gastroenterology and Hepatology 27: 888–892.

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  30. Giriwono, P.E., H. Shirakawa, H. Hokazono, T. Goto, and M. Komai. 2011. Fermented barley extract supplementation maintained antioxidative defense suppressing lipopolysaccharide-induced inflammatory liver injury in rats. Bioscience, Biotechnology, and Biochemistry 75: 1971–1976.

    Article  PubMed  CAS  Google Scholar 

  31. Gad, M., P. Ravn, D.A. Soborg, K. Lund-Jensen, A.C. Ouwehand, and S.S. Jensen. 2011. Regulation of the IL-10/IL-12 axis in human dendritic cells with probiotic bacteria. FEMS Immunology and Medical Microbiology 63: 93–107.

    Article  PubMed  CAS  Google Scholar 

  32. Mendis, E., M.M. Kim, N. Rajapakse, and S.K. Kim. 2008. Suppression of cytokine production in lipopolysaccharide-stimulated mouse macrophages by novel cationic glucosamine derivative involves down-regulation of NF-kappaB and MAPK expressions. Bioorganic and Medicinal Chemistry 16: 8390–8396.

    Article  PubMed  CAS  Google Scholar 

  33. Bengmark, S. 2006. Curcumin, an atoxic antioxidant and natural NFkappaB, cyclooxygenase-2, lipooxygenase, and inducible nitric oxide synthase inhibitor: a shield against acute and chronic diseases. JPEN Journal of Parenteral and Enteral Nutrition 30: 45–51.

    Article  PubMed  CAS  Google Scholar 

  34. McCabe, C., A. Samali, and T. O'Brien. 2006. Beta cell cytoprotective strategies: establishing the relative roles for iNOS and ROS. Biochemical and Biophysical Research Communications 342: 1240–1248.

    Article  PubMed  CAS  Google Scholar 

  35. Herath, T.D., R.P. Darveau, C.J. Seneviratne, C.Y. Wang, Y. Wang, and L. Jin. 2013. Tetra- and penta-acylated lipid A structures of Porphyromonas gingivalis LPS differentially activate TLR4-mediated NF-kappaB signal transduction cascade and immuno-inflammatory response in human gingival fibroblasts. PLoS One 8: e58496.

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  36. Joo, H.M., Y.J. Hyun, K.S. Myoung, Y.T. Ahn, J.H. Lee, C.S. Huh, M.J. Han, and D.H. Kim. 2011. Lactobacillus johnsonii HY7042 ameliorates Gardnerella vaginalis-induced vaginosis by killing Gardnerella vaginalis and inhibiting NF-kappaB activation. International Immunopharmacology 11: 1758–1765.

    Article  PubMed  CAS  Google Scholar 

  37. Shimazu, T., J. Villena, M. Tohno, H. Fujie, S. Hosoya, T. Shimosato, H. Aso, Y. Suda, Y. Kawai, T. Saito, S. Makino, S. Ikegami, H. Itoh, and H. Kitazawa. 2012. Immunobiotic Lactobacillus jensenii elicits anti-inflammatory activity in porcine intestinal epithelial cells by modulating negative regulators of the Toll-like receptor signaling pathway. Infection and Immunity 80: 276–288.

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  38. Wang, H., and Y. Li. 2006. Protective effect of bicyclol on acute hepatic failure induced by lipopolysaccharide and D-galactosamine in mice. European Journal of Pharmacology 534: 194–201.

    Article  PubMed  CAS  Google Scholar 

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Acknowledgments

This study was supported by the National Science and Technology Project (grant number 2011AA100902), the Science and Technology Project for the Universities of Shandong Province (grant number J13LE55), the National Natural Science Foundation of China (grant number 31171718), and the China Postdoctoral Science Foundation (grant number 2012 M510911).

Conflict of Interest

The authors declare that there are no conflicts of interest.

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Authors and Affiliations

  1. Key Laboratory of Dairy Science, Ministry of Education, Department of Food Science, Northeast Agricultural University, Harbin, Heilongjiang, 150030, China

    Xinyan Peng & Yujun Jiang

  2. National Research Center of Dairy Engineering and Technology, Northeast Agricultural University, Harbin, Heilongjiang, 150086, China

    Xinyan Peng & Yujun Jiang

  3. College of Food Engineering, Ludong University, Yantai, Shandong, 264025, China

    Xinyan Peng

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Correspondence to Yujun Jiang.

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Peng, X., Jiang, Y. Protective Effects of Lactobacillus plantarum NDC 75017 Against Lipopolysaccharide-Induced Liver Injury in Mice. Inflammation 37, 1599–1607 (2014). https://doi.org/10.1007/s10753-014-9886-1

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