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Noninvasive Allograft Imaging of Acute Rejection: Evaluation of 131I-anti-CXCL10 mAb

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Abstract

The purpose of this study was to investigate the use of iodine-131-labeled anti-CXCL10 mAb as tracer targeted at CXCL10 to detect acute rejection (AR) with mice model. Expression of CXCL10 was proved by RT-PCR, ELISA, and immunochemistry staining. All groups were submitted to whole-body autoradioimaging and ex vivo biodistribution studies after tail vein injection of 131I-anti-CXCL10 mAb. The highest concentration/expression of CXCL10 was detected in allograft tissue compared with allograft treated with tacrolimus and isograft control. Tacrolimus could obviously inhibit the rejection of allograft. Allograft could be obviously imaged at all checking points, much clearer than the other two groups. The biodistribution results showed the highest uptake of radiotracer in allograft. T/NT (target/nontarget) ratio was 4.15 ± 0.25 at 72 h, apparently different from allograft treated with tacrolimus (2.29 ± 0.10), P < 0.05. These data suggest that CXCL10 is a promising target for early stage AR imaging and 131I-CXCL10 mAb can successfully image AR and monitor the effect of immunosuppressant.

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Acknowledgments

This work was supported by grants from the National Natural Science Foundation of China (81371601, G, Hou).

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Authors and Affiliations

  1. Key Laboratory for Experimental Teratology of the Ministry of Education and Biomedical Isotope Research Center, School of Medicine, Shandong University, 44# Wenhua Xi Road, Jinan, Shandong, 250012, People’s Republic of China

    Dayan Cheng, Hukui Sun, Ting Liang, Chao Zhang, Jing Song & Guihua Hou

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  1. Dayan Cheng
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  2. Hukui Sun
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  3. Ting Liang
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  4. Chao Zhang
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  6. Guihua Hou
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Correspondence to Guihua Hou.

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Cheng, D., Sun, H., Liang, T. et al. Noninvasive Allograft Imaging of Acute Rejection: Evaluation of 131I-anti-CXCL10 mAb. Inflammation 38, 456–464 (2015). https://doi.org/10.1007/s10753-014-0050-8

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  • DOI: https://doi.org/10.1007/s10753-014-0050-8

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