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RETRACTED ARTICLE: Inhibition of Lipopolysaccharide (LPS)-Induced Inflammatory Responses by Selenium in Bovine Mammary Epithelial Cells in Primary Culture

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This article was retracted on 26 February 2015

Abstract

Selenium, in the form of selenoproteins, plays a pivotal role in anti-inflammatory processes and antioxidant defense system. The aim of this study was to examine the effects of selenium on lipopolysaccharide (LPS)-induced inflammatory responses in bovine mammary epithelial cells (bMEC) and to investigate the potential mechanism. bMEC viability was measured by MTT assay. TNF-α, IL-1β, cyclooxygenase-2 (COX-2), and inducible nitric oxide synthase (iNOS) messenger RNA (mRNA) expressions were evaluated by quantitative real-time polymerase chain reaction (qRT-PCR). The activation of nuclear factor-kappa B (NF-κB) was determined by Western blotting. The results showed that the mRNA expressions of these inflammatory factors were significantly inhibited by selenium in a dose-dependent manner. At protein levels, Western blot analysis demonstrated that selenium dose-dependently decreased NF-κB p65 translocating from the cytoplasm to the nucleus. Taken together, these results suggest that the anti-inflammatory property of selenium in LPS-stimulated primary bMEC may be attributed to the downregulation of NF-κB activation.

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References

  1. Cavero, D., K.-H. Tölle, C. Henze, C. Buxadé, and J. Krieter. 2008. Mastitis detection in dairy cows by application of neural networks. Livestock Science 114: 280–286.

    Article  Google Scholar 

  2. Seegers, H., C. Fourichon, and F. Beaudeau. 2003. Production effects related to mastitis and mastitis economics in dairy cattle herds. Veterinary Research 34: 475–491.

    Article  PubMed  Google Scholar 

  3. Sharma, N., N. Singh, and M. Bhadwal. 2011. Relationship of somatic cell count and mastitis: an overview. Asian-Australasian Journal of Animal Sciences 24: 429–438.

    Article  Google Scholar 

  4. Ibeagha-Awemu, E.M., J.-W. Lee, A.E. Ibeagha, D.D. Bannerman, M.J. Paape, and X. Zhao. 2008. Bacterial lipopolysaccharide induces increased expression of toll-like receptor (TLR) 4 and downstream TLR signaling molecules in bovine mammary epithelial cells. Veterinary Research 39: 1–12.

    Article  CAS  Google Scholar 

  5. Hoffmann, P.R., and M.J. Berry. 2008. The influence of selenium on immune responses. Molecular Nutrition & Food Research 52: 1273–1280.

    Article  CAS  Google Scholar 

  6. Duntas, L. 2009. Selenium and inflammation: underlying anti-inflammatory mechanisms. Hormone And Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Metabolisme 41: 443–447.

    Article  CAS  PubMed  Google Scholar 

  7. Guo, F., N. Monsefi, A. Moritz, and A. Beiras-Fernandez. 2012. Selenium and cardiovascular surgery: an overview. Current Drug Safety 7: 321–327.

    Article  CAS  PubMed  Google Scholar 

  8. Miggiano, G., and L. Gagliardi. 2004. Diet, nutrition and rheumatoid arthritis. La Clinica Terapeutica 156: 115–123.

    Google Scholar 

  9. Kravtsiv, R., A. Stadnyk, and M. Lychuk. 2003. Antioxidant vitamins and selenium in the prevention of white muscle disease in calves. Ukrainskiĭ Biokhimicheskiĭ Zhurnal 76: 90–99.

    Google Scholar 

  10. Zamamiri-Davis, F., Y. Lu, J.T. Thompson, K.S. Prabhu, P.V. Reddy, L.M. Sordillo, et al. 2002. Nuclear factor-κB mediates over-expression of cyclooxygenase-2 during activation of RAW 264.7 macrophages in selenium deficiency. Free Radical Biology & Medicine 32: 890–897.

    Article  CAS  Google Scholar 

  11. Prabhu, K., F. Zamamiri-Davis, J. Stewart, J. Thompson, L. Sordillo, and C. Reddy. 2002. Selenium deficiency increases the expression of inducible nitric oxide synthase in RAW 264.7 macrophages: role of nuclear factor-κB in up-regulation. Biochemical Journal 366: 203–209.

    PubMed Central  CAS  PubMed  Google Scholar 

  12. Zhang W, Zhang R, Wang T, Jiang H, Guo M, Zhou E, et al. 2013. Selenium inhibits LPS-induced pro-inflammatory gene expression by modulating MAPK and NF-κB signaling pathways in mouse mammary epithelial cells in primary culture. Inflammation 1–8.

  13. Fu, Y., B. Liu, X. Feng, Z. Liu, D. Liang, F. Li, et al. 2013. Lipopolysaccharide increases Toll-like receptor 4 and downstream Toll-like receptor signaling molecules expression in bovine endometrial epithelial cells. Veterinary Immunology and Immunopathology 151: 20–27.

    Article  CAS  PubMed  Google Scholar 

  14. Fu, Y., B. Liu, N. Zhang, Z. Liu, D. Liang, F. Li, et al. 2013. Magnolol inhibits lipopolysaccharide-induced inflammatory response by interfering with TLR4 mediated NF-κB and MAPKs signaling pathways. Journal of Ethnopharmacology 145: 193–199.

    Article  CAS  PubMed  Google Scholar 

  15. Rossol M, Heine H, Meusch U, Quandt D, Klein C, Sweet MJ, et al. 2011. LPS-induced cytokine production in human monocytes and macrophages. Critical Reviews™ in Immunology 31.

  16. Andrieu, S. 2008. Is there a role for organic trace element supplements in transition cow health? The Veterinary Journal 176: 77–83.

    Article  CAS  PubMed  Google Scholar 

  17. Spears, J.W., and W.P. Weiss. 2008. Role of antioxidants and trace elements in health and immunity of transition dairy cows. The Veterinary Journal 176: 70–76.

    Article  CAS  PubMed  Google Scholar 

  18. Rayman, M.P. 2012. Selenium and human health. The Lancet 379: 1256–1268.

    Article  CAS  Google Scholar 

  19. Lee, J.-W., D.D. Bannerman, M.J. Paape, M.-K. Huang, and X. Zhao. 2006. Characterization of cytokine expression in milk somatic cells during intramammary infections with Escherichia coli or Staphylococcus aureus by real-time PCR. Veterinary Research 37: 219–229.

    Article  CAS  PubMed  Google Scholar 

  20. Hwang, P.-A., S.-Y. Chien, Y.-L. Chan, M.-K. Lu, C.-H. Wu, Z.-L. Kong, et al. 2011. Inhibition of lipopolysaccharide (LPS)-induced inflammatory responses by Sargassum hemiphyllum sulfated polysaccharide extract in RAW 264.7 macrophage cells. Journal of Agricultural and Food Chemistry 59: 2062–2068.

    Article  CAS  PubMed  Google Scholar 

  21. Buraczynska, M., P. Ksiazek, P. Kubit, and W. Zaluska. 2006. Interleukin-1 receptor antagonist gene polymorphism affects the progression of chronic renal failure. Cytokine 36: 167–172.

    Article  CAS  PubMed  Google Scholar 

  22. Oviedo-Boyso, J., J.J. Valdez-Alarcon, M. Cajero-Juarez, A. Ochoa-Zarzosa, J.E. Lopez-Meza, A. Bravo-Patino, et al. 2007. Innate immune response of bovine mammary gland to pathogenic bacteria responsible for mastitis. The Journal of Infection 54: 399–409.

    Article  PubMed  Google Scholar 

  23. Li, D., N. Zhang, Y. Cao, W. Zhang, G. Su, Y. Sun, et al. 2013. Emodin ameliorates lipopolysaccharide-induced mastitis in mice by inhibiting activation of NF-kappaB and MAPKs signal pathways. European Journal of Pharmacology 705: 79–85.

    Article  CAS  PubMed  Google Scholar 

  24. Smith, W.L., and R. Langenbach. 2001. Why there are two cyclooxygenase isozymes. Journal of Clinical Investigation 107: 1491–1495.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  25. Feng, L., Y. Xia, G. Garcia, D. Hwang, and C. Wilson. 1995. Involvement of reactive oxygen intermediates in cyclooxygenase-2 expression induced by interleukin-1, tumor necrosis factor-alpha, and lipopolysaccharide. Journal of Clinical Investigation 95: 1669.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  26. Cho, H., C.-W. Yun, W.-K. Park, J.-Y. Kong, K.S. Kim, Y. Park, et al. 2004. Modulation of the activity of pro-inflammatory enzymes, COX-2 and iNOS, by chrysin derivatives. Pharmacological Research 49: 37–43.

    Article  CAS  PubMed  Google Scholar 

  27. Vunta, H., B.J. Belda, R.J. Arner, C. Channa Reddy, J.P. Vanden Heuvel, and P.K. Sandeep. 2008. Selenium attenuates pro‐inflammatory gene expression in macrophages. Molecular Nutrition & Food Research 52: 1316–1323.

    Article  CAS  Google Scholar 

  28. Lawrence, T., D.W. Gilroy, P.R. Colville-Nash, and D.A. Willoughby. 2001. Possible new role for NF-κB in the resolution of inflammation. Nature Medicine 7: 1291–1297.

    Article  CAS  PubMed  Google Scholar 

  29. Zhang, G., and S. Ghosh. 2001. Toll-like receptor–mediated NF-κB activation: a phylogenetically conserved paradigm in innate immunity. Journal of Clinical Investigation 107: 13–19.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  30. Pantano, C., N.L. Reynaert, A.V.D. Vliet, and Y.M. Janssen–Heininger. 2006. Redox-sensitive kinases of the nuclear factor-κB signaling pathway. Antioxidants & Redox Signaling 8: 1791–1806.

    Article  CAS  Google Scholar 

  31. Vunta, H., F. Davis, U.D. Palempalli, D. Bhat, R.J. Arner, J.T. Thompson, et al. 2007. The anti-inflammatory effects of selenium are mediated through 15-deoxy-Δ12, 14-prostaglandin J2 in macrophages. Journal of Biological Chemistry 282: 17964–17973.

    Article  CAS  PubMed  Google Scholar 

  32. Ricote, M., A.C. Li, T.M. Willson, C.J. Kelly, and C.K. Glass. 1998. The peroxisome proliferator-activated receptor-γ is a negative regulator of macrophage activation. Nature 391: 79–82.

    Article  CAS  PubMed  Google Scholar 

  33. Pascual, G., A.L. Fong, S. Ogawa, A. Gamliel, A.C. Li, V. Perissi, et al. 2005. A SUMOylation-dependent pathway mediates transrepression of inflammatory response genes by PPAR-γ. Nature 437: 759–763.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

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Acknowledgments

This study was supported by China Postdoctoral Science Foundation (2013 M540255).

Author information

Authors and Affiliations

  1. College of Veterinary Medicine, Jilin University, Changchun, 130062, Jilin Province, China

    Zhengkai Wei, Minjun Yao, Yimeng Li & Zhengtao Yang

  2. Guangdong Haid Institute of Animal Husbandry and Veterinary, Guangzhou, 510000, Guangdong, China

    Xiaosheng Feng

Authors
  1. Zhengkai Wei
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  2. Minjun Yao
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  3. Yimeng Li
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  4. Zhengtao Yang
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  5. Xiaosheng Feng
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Corresponding author

Correspondence to Xiaosheng Feng.

Additional information

Zhengkai Wei and Minjun Yao contributed equally to this work.

The authors hereby retract the article entitled “Inhibition of Lipopolysaccharide (LPS)-Induced Inflammatory Responses by Selenium in Bovine Mammary Epithelial Cells in Primary Culture” (Wei et al. 2014) published in Inflammation, Volume 38 / Issue 1 (February 2015) because of inappropriate statistical analysis and inability to reproduce some of the results.

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Wei, Z., Yao, M., Li, Y. et al. RETRACTED ARTICLE: Inhibition of Lipopolysaccharide (LPS)-Induced Inflammatory Responses by Selenium in Bovine Mammary Epithelial Cells in Primary Culture. Inflammation 38, 152–158 (2015). https://doi.org/10.1007/s10753-014-0017-9

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  • DOI: https://doi.org/10.1007/s10753-014-0017-9

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