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Pemetrexed Ameliorates Experimental Arthritis in Rats

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Abstract

Pemetrexed (PMTX) is an anti-folate drug as methotrexate. The purpose of this study was to assess the efficacy of PMTX on collagen-induced arthritis (CIA). Forty Wistar albino rats were randomized into four groups. Arthritis was induced by intradermal injection of chicken type II collagen combined with incomplete Freund’s adjuvant. Animals were sacrificed at the 15th day after the onset of arthritis. Tumor necrosis factor alpha (TNF-α), interleukin (IL)-17, and malondialdehyde (MDA) levels were increased, and superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) activities and the expressions of nuclear factor erythroid 2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) were decreased in the arthritis group. In the PMTX-treated (0.2 and 1 mg/kg/week i.p.) groups, the levels of TNF-α, IL-17, and MDA were decreased; the activities of SOD, CAT, and GPx and the expressions of Nrf2 and HO-1 were restored, and perisynovial inflammation and cartilage–bone destruction were decreased. PMTX has anti-arthritic potential in the CIA model and may be a therapeutic agent for rheumatoid arthritis.

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Approval was obtained from the Ethics Committee of Firat University.

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Authors and Affiliations

  1. Department of Rheumatology, Faculty of Medicine, Firat University, Elazig, Turkey

    Ahmet Karatas, Suleyman Serdar Koca & Ahmet Isik

  2. Department of Rheumatology, Faculty of Medicine, 19 Mayis University, Samsun, Turkey

    Metin Ozgen

  3. Department of Pathology, Faculty of Medicine, Firat University, Elazig, Turkey

    Adile Ferda Dagli

  4. School of Health Sciences, Firat University, Elazig, Turkey

    Fazilet Erman

  5. Department of Animal Nutrition, Faculty of Veterinary Science, Firat University, Elazig, Turkey

    Nuran Sahin & Kazim Sahin

  6. Firat Universitesi, Tip Fakultesi, Romatoloji BD., 23119, Elazig, Turkey

    Suleyman Serdar Koca

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Correspondence to Suleyman Serdar Koca.

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Karatas, A., Koca, S.S., Ozgen, M. et al. Pemetrexed Ameliorates Experimental Arthritis in Rats. Inflammation 38, 9–15 (2015). https://doi.org/10.1007/s10753-014-0002-3

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  • DOI: https://doi.org/10.1007/s10753-014-0002-3

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