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Sca-1-Positive Cardiac Stem Cell migration in a Cardiac Infarction Model

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A Correction to this article was published on 12 August 2020

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Abstract

Adult myocardium has the capacity for repair and regeneration, which is derived from cardiac stem cells (CSCs). In this study, we assessed the migration and changes in numbers of Sca-1-positive CSCs after myocardial infarction (MI) in vivo and in vitro. In this study, we showed that in a rat MI model the CSCs emerged around the vessels near the peri-infarct zone and in the epicardium of the infarcted area. Four weeks after infarction, no differences in the expression of connexin 43 (Cx43) were observed in the peri-infarct and infarct zones. In vitro, we mimicked tissue ischemia and hypoxia by using a culture environment of 5 % O2 and a wound healing assay to monitor the migration of CSCs. In conclusion, under hypoxic conditions, the CSCs, conveyed by blood vessels, migrated from the niche to the infarct zone for repairing the damaged myocytes. The number of endogenous migrating CSCs was proportionate to the repair time after infarction, rather than the degree of infarction. Four weeks after MI, the expression of Cx43 was not altered in migratory CSCs, namely no enhanced gap-junctional communication with cardiomyocytes was seen in the CSCs. Further studies are necessary to delineate the molecular mechanisms that drive the migration of CSCs after MI.

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Change history

  • 12 August 2020

    After publication of our article [1], we became aware that there were errors in Fig. 1, namely that the Echocardiographic assessment of 1w, 2w, and 4w groups was incorrectly presented.

Abbreviations

CSC:

cardiac stem cell

MI:

myocardial infarction

AMI:

acute myocardial infarction

LAD:

left anterior descending coronary artery

MACS:

magnetic cell sorting

LV:

left ventricle

LVEF:

left ventricular ejection fraction

LVFS:

left ventricular fractional shortening

Cx43:

connexin 43

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Acknowledgments

This study was supported in part by a grant from the National Natural Science Foundation of China (to B.Y., Grant No. 30871064). The authors thank Baixiang Li, Wei Liu and Hulun Li for their helps with technical assistance, and also thank Dr. Meng Sun for assistance with the statistics.

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Authors and Affiliations

  1. Key Laboratories of Education Ministry for Myocardial Ischemia Mechanism and Treatment, Harbin, China

    Jingjin Liu, Yongshun Wang, Wenjuan Du & Bo Yu

  2. Depatment of Cardiology, Second Affiliated Hospital of Harbin Medical University, 150086, Harbin, China

    Jingjin Liu, Yongshun Wang, Wenjuan Du & Bo Yu

Authors
  1. Jingjin Liu
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  2. Yongshun Wang
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  3. Wenjuan Du
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  4. Bo Yu
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Corresponding author

Correspondence to Bo Yu.

Additional information

J. Liu and Y. Wang contributed equally to this work.

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Liu, J., Wang, Y., Du, W. et al. Sca-1-Positive Cardiac Stem Cell migration in a Cardiac Infarction Model. Inflammation 36, 738–749 (2013). https://doi.org/10.1007/s10753-013-9600-8

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  • DOI: https://doi.org/10.1007/s10753-013-9600-8

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