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Inhibitory Mechanism of 10-Hydroxy-trans-2-decenoic Acid (Royal Jelly Acid) Against Lipopolysaccharide- and Interferon-β-Induced Nitric Oxide Production

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Abstract

Royal jelly acid, 10-hydroxy-trans-2-decenoic acid (10H2DA), is a major lipid component of royal jelly, which is the exclusive diet of queen honeybees. Previously, we showed partial inhibition of lipopolysaccharide (LPS)-induced NF-κB activation by 10H2DA. In this study, the ability of 10H2DA to inhibit LPS-induced nitric oxide (NO) production was investigated. LPS-induced NO production and inducible NO synthase (iNOS) gene transcription were inhibited by 10H2DA. LPS-stimulated interferon (IFN)-β production, IFN regulatory factor-1 induction and IFN-stimulated response element activation, which are required for iNOS induction, were unaffected by 10H2DA. IFN-β-induced NO production, however, was significantly inhibited by 10H2DA. Furthermore, IFN-β-induced nuclear factor (NF)-κB activation and tumour necrosis factor (TNF)-α production were significantly inhibited by 10H2DA, and TNF-α-induced NF-κB activation was also inhibited by 10H2DA. These results and our previous study suggest that 10H2DA inhibits LPS- and IFN-β-induced NO production via inhibition of NF-κB activation induced by LPS or IFN-β.

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Acknowledgments

This work was supported by a grant from the Japan Royal Jelly Fair Trade Council (Tokyo, Japan).

Conflict of Interest

The authors have no conflict of interest to declare.

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Authors and Affiliations

  1. Department of Biopharmaceutical Sciences, Laboratory of Microbiology, Gifu Pharmaceutical University, 1-25-4 Daigaku-Nishi, Gifu, 501-1196, Japan

    Tsuyoshi Sugiyama, Keita Takahashi, Akihiro Kuzumaki, Shunji Tokoro, Paola Neri & Hiroshi Mori

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  1. Tsuyoshi Sugiyama
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  2. Keita Takahashi
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  3. Akihiro Kuzumaki
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  4. Shunji Tokoro
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  6. Hiroshi Mori
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Correspondence to Tsuyoshi Sugiyama.

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Sugiyama, T., Takahashi, K., Kuzumaki, A. et al. Inhibitory Mechanism of 10-Hydroxy-trans-2-decenoic Acid (Royal Jelly Acid) Against Lipopolysaccharide- and Interferon-β-Induced Nitric Oxide Production. Inflammation 36, 372–378 (2013). https://doi.org/10.1007/s10753-012-9556-0

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