Inflammation

, 31:215

Hepatoprotective Effect of Infliximab, an Anti-TNF-α Agent, on Carbon Tetrachloride-Induced Hepatic Fibrosis

Authors

  • Ibrahim Halil Bahcecioglu
    • Department of Gastroenterology, Faculty of MedicineFırat University
    • Department of Internal Medicine and Rheumatology, Faculty of MedicineFırat University
    • Tıp Fakultesi, RomatolojiFırat universitesi
  • Orhan Kursat Poyrazoglu
    • Department of Gastroenterology, Faculty of MedicineFırat University
  • Mehmet Yalniz
    • Department of Gastroenterology, Faculty of MedicineFırat University
  • Ibrahim Hanifi Ozercan
    • Department of Pathology, Faculty of MedicineFırat University
  • Bilal Ustundag
    • Department of Biochemistry, Faculty of MedicineFırat University
  • Kazim Sahin
    • Department of Animal Nutrition, Faculty of VeterinaryFırat University
  • Adile Ferda Dagli
    • Department of Pathology, Faculty of MedicineFırat University
  • Ahmet Isik
    • Department of Internal Medicine and Rheumatology, Faculty of MedicineFırat University
Article

DOI: 10.1007/s10753-008-9067-1

Cite this article as:
Bahcecioglu, I.H., Koca, S.S., Poyrazoglu, O.K. et al. Inflammation (2008) 31: 215. doi:10.1007/s10753-008-9067-1

Abstract

To assess the effect of infliximab, an anti-tumor necrosis factor (TNF)-α agent, on the carbon tetrachloride (CCl4)-induced hepatic fibrosis in rats. Rats were randomized into three groups (n = 9). The control group received only intraperitoneal (i.p.) olive oil. Hepatic fibrosis was induced by repeated i.p. injections of 1.5 ml/kg CCl4 (1:3 mixture with olive oil) for 5 weeks in the remaining two groups which were also injected subcutaneous saline or 2 mg/kg infliximab. Infliximab reduced the levels of aspartate aminotransferase and alanine aminotransferase (p < 0.05 for both). The scores of hepatic necrosis, inflammation and fibrosis, and expression of α-smooth muscle actin were lower in the infliximab-treated group than the CCI4-treated group (p < 0.01, p < 0.001, p < 0.01, p < 0.001, respectively). However, there was no significant difference in terms of liver tissue and plasma malondialdehyde, and serum TNF-α levels, while infliximab relatively reduced the level of transforming growth factor-β1 (373.0 ± 153.1 vs. 280.8 ± 127.1 pg/ml). Treatment with infliximab attenuated the necro-inflammation and fibrogenesis in the CCI4-induced hepatic fibrosis, and thus it might be effective as a therapeutic anti-fibrotic agent.

KEY WORDS

carbon tetrachloridehepatic fibrosisinfliximab

Copyright information

© Springer Science+Business Media, LLC 2008