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Out with the old, in with the new? Younger Daphnia clones are competitively superior over centuries-old ancestors

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Abstract

A laboratory microcosm experiment was conducted to examine the roles of food quantity and food quality on the competitive abilities of clones of the keystone aquatic zooplankter, Daphnia pulicaria. Using methods of resurrection ecology, clones were established by hatching dormant eggs from sediment layers of a lake (South Center, MN, U.S.A) that were separated by centuries of environmental change (nutrient enrichment). Two sets of paired clones (“modern” vs. “ancient”) were competed against each other in a 2 × 2 factorial design of high/low food quantity and high/low food quality. Experimental carbon:phosphorus (C:P) ratios were designed to mimic food quantity and food quality parameters respective to each of the two time periods from which the clones were extracted to test whether clones are competitively superior under conditions representative of the time period in which they existed. Contrary to predictions, we found that “modern” clones were able to outcompete “ancient” clones under all food treatments. We discuss potential mechanisms (i.e., mutational input, viability of long-dormant eggs, differences in phenotypic plasticity) that may have differentially impacted the performance of experimental clones. Our results should aid in deciphering microevolutionary dynamics observed in resurrection ecology studies, a powerful tool that can illuminate adaptive dynamics of organisms to environmental changes on long-term (centuries-long) temporal scales.

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References

  • Baird, N. A., P. D. Etter, T. S. Atwood, M. C. Currey, A. L. Shiver, Z. A. Lewis, E. U. Selker, W. A. Cresko & E. A. Johnson, 2008. Rapid SNP discovery and genetic mapping using sequenced RAD markers. PLoS One 3: e3376.

    Article  PubMed Central  PubMed  Google Scholar 

  • Bennington, C. C., J. B. McGraw & M. C. Varek, 1991. Ecological genetic variation in seed banks. II. Phenotypic and genetic differences between young and old subpopulations of Luzula parviflora. Journal of Ecology 79: 627–643.

    Article  Google Scholar 

  • Cáceres, C. E., 1998. Interspecific variation in the abundance, production and emergence of Daphnia diapausing eggs. Ecology 79: 1699–1710.

    Article  Google Scholar 

  • Cheah, K. S. E. & D. J. Osborne, 1978. DNA lesions occur with loss of viability in embryos of aging rye seed. Nature 272: 593–599.

    Article  CAS  PubMed  Google Scholar 

  • Chwedorzewska, K. J., P. T. Bednarek, R. Lewandowska, P. Krajewski & J. Puchalski, 2006. Studies on genetic changes in rye samples (Secale cereale L.) maintained in a seed bank. Cellular & Molecular Biology Letters 11: 338–347.

    Article  CAS  Google Scholar 

  • Cousyn, C., L. De Meester, J. K. Colbourne, L. Brendonck, D. Verschuren & F. Volckaert, 2001. Rapid, local adaptation of zooplankton behavior to changes in predation pressure in the absence of neutral genetic changes. Proceedings of the National Academy of Sciences, U.S.A. 98: 6256–6260.

  • Decaestecker, E., S. Gaba, J. A. M. Raeymaekers, R. Stoks, L. Van Kerckhoven, D. Ebert & L. De Meester, 2007. Host-parasite ‘Red Queen’ dynamics archived in pond sediment. Nature 450: 870–874.

    Article  CAS  PubMed  Google Scholar 

  • DeMott, W. R., R. D. Gulati & K. Siewertsen, 1998. Effects of phosphorus-deficient diets on the carbon and phosphorus balance of Daphnia magna. Limnology and Oceanography 43: 1147–1161.

    Article  CAS  Google Scholar 

  • Frisch, D., P. M. Morton, P. Roy Chowdhury, B. W. Culver, J. K. Colbourne, L. J. Weider & P. D. Jeyasingh, 2014. A millennial-scale chronicle of evolutionary responses to cultural eutrophication in Daphnia. Ecology Letters 17: 360–368.

    Article  PubMed  Google Scholar 

  • Grover, J. P., 1997. Resource competition. Chapman & Hall, New York.

    Book  Google Scholar 

  • Hairston Jr., N. G., R. A. Van Brunt, C. M. Kearns & D. R. Engstrom, 1995. Age and survivorship of diapausing eggs in a sediment egg bank. Ecology 76: 1706–1711.

    Article  Google Scholar 

  • Harmon, J. P., N. A. Moran & A. R. Ives, 2009. Species response to environmental change: impacts of food web interactions and evolution. Science 323: 1347–1350.

    Article  CAS  PubMed  Google Scholar 

  • Hebert, P. D. N. & M. J. Beaton, 1993. Methodologies for allozyme analysis using Cellulose acetate electrophoresis. A practical handbook. Helena Laboratories, Beaumont.

    Google Scholar 

  • Hedrick, P. W., 2006. Genetic polymorphisms in heterogenous environments: the age of genomics. Annual Review Ecology Evolution & Systematics 37: 67–93.

    Article  Google Scholar 

  • Jeyasingh, P. D., L. J. Weider & R. W. Sterner, 2009. Genetically-based trade-offs in response to stoichiometric food quality influence competition in a keystone aquatic herbivore. Ecology Letters 12: 1229–1237.

    Article  PubMed  Google Scholar 

  • Kareiva, P. M., J. G. Kingsolver & R. B. Huey, 1992. Biotic interactions and global change. Sinauer & Associates, Sunderland, MA.

    Google Scholar 

  • Kerfoot, W. C. & L. J. Weider, 2004. Experimental paleoecology (resurrection ecology): chasing Van Valen’s Red Queen hypothesis. Limnology & Oceanography 49: 1300–1316.

    Article  Google Scholar 

  • Kerfoot, W. C., J. A. Robbins & L. J. Weider, 1999. A new approach to historical reconstruction: combining descriptive and experimental paleolimnology. Limnology & Oceanography 44: 1232–1247.

    Article  Google Scholar 

  • Kilham, S. S., D. A. Kreeger, S. G. Lynn, C. E. Goulden & L. Herrera, 1998. COMBO: a defined freshwater culture medium for algae and zooplankton. Hydrobiologia 377: 147–159.

    Article  CAS  Google Scholar 

  • Levin, D. A., 1990. The seed bank as a source of genetic novelty in plants. American Naturalist 135: 563–572.

    Article  Google Scholar 

  • Marcus, N. H., R. Lutz, W. Burnett & P. Cable, 1994. Age, viability and vertical distribution of zooplankton resting eggs from an anoxic basin: evidence of an egg bank. Limnology & Oceanography 39: 154–158.

    Article  Google Scholar 

  • McGraw, J. B., 1993. Ecological genetic variation in seed banks. IV. Differentiation of extant and seed bank-derived populations of Eriophorum vaginatum. Arctic & Alpine Research 25: 45–49.

    Article  Google Scholar 

  • Minnesota Pollution Control Agency, 2011. Sentinel Lake Assessment Report South Center Lake (13-0027) Chisago County, Minnesota. St. Paul, MN, http://www.pca.state.mn.us.

  • Orsini, L., K. Schwenk, L. De Meester, J. K. Colbourne, M. E. Pfrender & L. J. Weider, 2013. The evolutionary time machine: using dormant propagules to forecast how populations can adapt to changing environments. Trends in Ecology & Evolution 28: 274–282.

    Article  Google Scholar 

  • Schoen, D. J., J. L. David & T. M. Bataillon, 1998. Deleterious mutation accumulation and the regeneration of genetic resources. Proceedings of the National Academy of Sciences USA 95: 394–399.

    Article  CAS  Google Scholar 

  • Tilman, D., 1982. Resource competition and community structure. Princeton University Press, Princeton.

    Google Scholar 

  • Vavrek, M. C., J. B. McGraw & C. C. Bennington, 1991. Ecological genetic variation in seed banks. III. Phenotypic and genetic differences between young and old seed populations of Carex bigelowii. Journal of Ecology 79: 645–662.

    Article  Google Scholar 

  • Visser, M., 2008. Keeping up with a warming world; assessing the rate of adaptation to climate change. Proceedings of the Royal Society London B 275: 649–659.

    Article  Google Scholar 

  • Weider, L. J., W. Lampert, M. Wessels, J. K. Colbourne & P. Limburg, 1997. Long-term genetic shifts in a microcrustacean egg bank associated with anthropogenic changes in the Lake Constance ecosystem. Proceedings of the Royal Society London B 264: 1613–1618.

    Article  Google Scholar 

  • Weider, L. J., W. Makino, K. Acharya, K. L. Glenn, M. Kyle, J. Urabe & J. J. Elser, 2005. Genotype x environment interactions, stoichiometric food quality effects, and clonal coexistence in Daphnia pulex. Oecologia 143: 537–547.

    Article  PubMed  Google Scholar 

  • Wolf, H. G. & L. J. Weider, 1991. Do life-history parameters of Daphnia as determined in the laboratory correctly predict species successions in the field? Verhandlungen des Internationalen Verein Limnologie 24: 2799–2801.

    Google Scholar 

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Acknowledgments

Funding for this study was provided by the U.S. National Science Foundation (NSF-IOS-OEI) collaborative Grants #0924289 and #1256881 to L.J.W. and Grant #0924401 to P.D.J. We thank the staff at the U. of Minnesota LacCORE facility, in particular R. O’Grady, for assisting with logistics during the field sampling. We thank B. Culver for lab assistance. We thank three anonymous reviewers for constructive comments that improved our manuscript.

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Author notes

  1. Philip K. Morton

    Present address: Division of Science, Murray State College, Tishomingo, OK, USA

Authors and Affiliations

  1. University of Oklahoma Biological Station, Station Road, Kingston, OK, 15389, USA

    Philip K. Morton, Dagmar Frisch & Lawrence J. Weider

  2. Department of Integrative Biology, Oklahoma State University, Stillwater, OK, USA

    Punidan D. Jeyasingh

  3. Department of Biology, Program in Ecology & Evolutionary Biology, University of Oklahoma, Norman, OK, USA

    Lawrence J. Weider

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  1. Philip K. Morton
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  2. Dagmar Frisch
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  3. Punidan D. Jeyasingh
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  4. Lawrence J. Weider
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Correspondence to Lawrence J. Weider.

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Handling editor: John Havel

Philip K. Morton and Dagmar Frisch have contributed equally to this work.

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Morton, P.K., Frisch, D., Jeyasingh, P.D. et al. Out with the old, in with the new? Younger Daphnia clones are competitively superior over centuries-old ancestors. Hydrobiologia 749, 43–52 (2015). https://doi.org/10.1007/s10750-014-2145-5

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  • DOI: https://doi.org/10.1007/s10750-014-2145-5

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