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Overexpression of leucine aminopeptidase 3 contributes to malignant development of human esophageal squamous cell carcinoma

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Abstract

Leucine aminopeptidases (LAPs) were associated with tumor cell proliferation, invasion and/or angiogenesis. We aimed to examine the biological function of LAP3 in esophageal squamous cell carcinoma (ESCC). LAP3 expressions were examined in human ESCC tissue and cell lines ECA109 and TE1 cells. Recombinant pSilencer4.1-LAP3–shRNA was transfected into ECA109 cells to silence LAP3 expression. The effects of LAP3 silencing on ECA109 cell proliferation in vitro were evaluated. Flow cytometry profiling was used to detect the differentiate cell cycle distribution in LAP3-silenced ECA109 cells. Wound-healing assay and transwell assay were used to examine the activities of migration and invasion in LAP3-silenced ECA109 cells. We overexpressed LAP3 in TE1 cells to find out the corresponding results. LAP3 expression level was abundance in ESCC tissue. LAP3 silencing significantly reduced ECA109 cell proliferation and colony formation. The knockdown of LAP3 resulted in cell cycle arrest at G1-phase. Moreover, over expression of LAP3 favors TE1 cell proliferation and invasiveness which also confirms its contribution in malignant development. We came to the conclusion that LAP3 contributed to ESCC progression by overcoming cell cycle arrest. The proliferative and migration effects of LAP3 might contribute to malignant development of human ESCC.

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Conflict of interest

All the authors declare no conflict of interest.

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Authors and Affiliations

  1. Department of Pathology, Affiliated Hospital of Nantong University, Nantong, Jiangsu Province, 226001, People’s Republic of China

    Shu Zhang, Qun Xue, Hanru Ren & Jianguo Zhang

  2. Department of Medical Oncology, Affiliated Hospital of Nantong University, Nantong, Jiangsu Province, 226001, People’s Republic of China

    Xiaojing Yang & Mei Li

  3. Department of Immunology Medical College, Nantong University, Nantong, Jiangsu Province, 226001, People’s Republic of China

    Xiaojing Yang & Li Yao

  4. Department of Thoracic Surgery, Affiliated Hospital of Nantong University, Nantong, Jiangsu Province, 226001, People’s Republic of China

    Hui Shi

  5. Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, 201203, People’s Republic of China

    Xueyu Chen

  6. Department of Oncology, Shanghai Medical College, Fudan University, No 270 Dongan Road, Shanghai, 200032, People’s Republic of China

    Huijie Wang

Authors
  1. Shu Zhang
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  2. Xiaojing Yang
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  3. Hui Shi
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  4. Mei Li
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  5. Qun Xue
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  6. Hanru Ren
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  7. Li Yao
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  8. Xueyu Chen
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  9. Jianguo Zhang
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  10. Huijie Wang
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Corresponding authors

Correspondence to Jianguo Zhang or Huijie Wang.

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Shu Zhang and Xiaojing Yang have contributed equally to this work.

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Zhang, S., Yang, X., Shi, H. et al. Overexpression of leucine aminopeptidase 3 contributes to malignant development of human esophageal squamous cell carcinoma. J Mol Hist 45, 283–292 (2014). https://doi.org/10.1007/s10735-014-9566-3

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  • DOI: https://doi.org/10.1007/s10735-014-9566-3

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