Journal of Molecular Histology

, Volume 41, Issue 1, pp 81–87

Heart failure increases atrogin-1 and MuRF1 gene expression in skeletal muscle with fiber type-specific atrophy

Authors

    • Department of Morphology, Institute of BiosciencesUNESP São Paulo State University
  • Eduardo Paulino Castan
    • Department of Morphology, Institute of BiosciencesUNESP São Paulo State University
  • Cesar Augusto Coelho
    • Department of Morphology, Institute of BiosciencesUNESP São Paulo State University
  • Francis Silva Lopes
    • Department of PhysiotherapyUNOESTE
  • Fernanda Losi Alves Almeida
    • Department of Morphology, Institute of BiosciencesUNESP São Paulo State University
  • Aline Michelin
    • Department of Morphology, Institute of BiosciencesUNESP São Paulo State University
  • Rodrigo Wagner Alves de Souza
    • Department of Morphology, Institute of BiosciencesUNESP São Paulo State University
  • João Pessoa AraújoJr.
    • Department of Microbiology and Immunology, Institute of BiosciencesUNESP São Paulo State University
  • Antonio Carlos Cicogna
    • Department of Internal Medicine, School of MedicineUNESP São Paulo State University
  • Maeli Dal Pai-Silva
    • Department of Morphology, Institute of BiosciencesUNESP São Paulo State University
Brief Communication

DOI: 10.1007/s10735-010-9262-x

Cite this article as:
Carvalho, R.F., Castan, E.P., Coelho, C.A. et al. J Mol Hist (2010) 41: 81. doi:10.1007/s10735-010-9262-x

Abstract

Heart failure (HF) is characterized by a reduced tolerance to exercise due to early fatigue and dyspnea; this may be due in part to skeletal muscle myopathy with a shift from slow to fast fibers and loss of muscle mass. Muscle wasting does not occur similarly in all types of muscle fiber, thus we tested the hypothesis that HF induces skeletal muscle atrophy in a fiber type-specific manner altering the expression of atrogin-1 and MuRF1 in a fast muscle of rats with monocrotaline-induced heart failure. We studied extensor digitorum longus (EDL) muscle from both HF and control Wistar rats. Atrogin-1 and MuRF1 mRNA content were determined using Real-Time RT-qPCR while muscle fiber cross-sectional area (CSA) from sections stained histochemically for myofibrillar ATPase were used as an index of type-specific fiber atrophy. The measurement of gene expression by RT-qPCR revealed that EDL muscle mRNA expression of MuRF1 and atrogin-1 was significantly increased in the HF group. Muscle fiber type IIB CSA decreased in the HF group compared to the CT group; there was no significant difference in muscle fiber types I and IIA/D CSA between the HF and CT groups. In conclusion, we showed that HF induces fiber type IIB specific atrophy, up-regulating atrogin-1 and MuRF1 mRNA expression in EDL muscle of monocrotaline treated rats.

Keywords

Atrogin-1MuRF1Fiber Type-Specific Atrophy

Copyright information

© Springer Science+Business Media B.V. 2010