Skip to main content
Log in

A SNP in the promoter region of theVvmybA1 gene is responsible for differences in grape berry color between two related bud sports of grape

  • Original paper
  • Published:
Plant Growth Regulation Aims and scope Submit manuscript

Abstract

The VvmybA1 gene in grape (Vitis vinifera) plays a key role in the biosynthesis of anthocyanin. The grape cultivars, ‘Benitaka’ (red color) and ‘Brazil’ (black color) were the result of a bud mutation. ‘Benetika’ was derived from ‘Italia’ (green color) and ‘Brazil’ was developed from ‘Benetika’. Single sequence repeat (SSR) molecular marker analysis was performed in order to demonstrate that the three cultivars have a common pedigree. A sequence analysis of the promoter region and coding sequence of VvmybA1 revealed a base substitution between ‘Benitaka’ and ‘Brazil’ in the promoter region and a deletion of a large DNA fragment in the promoter region of ‘Italia’. Anthocyanin content and expression of the VvmybA1 and UFGT genes in ‘Brazil’ were higher than in ‘Benitaka’ and barely detectable in ‘Italia’. A transient expression system was used to introduce VvmybA1 driven by the three different promoters present in ‘Italia’, ‘Brazil’, and ‘Benitaka’ into somatic embryos of ‘Centennial Seedless’ (Vitis vinifera L.) by Agrobacterium-mediated transformation. This resulted in the production of red cells in the embryos transformed with the constructs of VvmybA1 from ‘Brazil’ and ‘Benitaka’ and no color production in embryos transformed with the VvmybA1 construct from ‘Italia’. In addition, the embryos transformed with the ‘Brazil’ construct had more red color than the embryos transformed with the ‘Benitaka’ construct. These results suggested that a SNP mutation in the promoter region of VvmybA1 in ‘Benitaka’ (red color) was responsible for the color change displayed by ‘Brazil’ (black color).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  • Aharoni A, De Vos CH, Wein M, Sun ZK, Greco R, Kroon A (2001) The strawberry FaMYB1 transcription factor suppresses anthocyanins and flavonol accumulation in transgenic tobacco. Plant J 28:319–332

    Article  CAS  PubMed  Google Scholar 

  • Azuma A, Kobayashi S, Goto-Yamamoto N, Shiraishi M, Mitani N, Yakushiji H, Koshita Y (2009) Color recovery in berries of grape (Vitis vinifera L.) ‘Benitaka’, a bud sport of ‘Italia’, is caused by a novel allele at the VvmybA1 locus. Plant Sci 176:470–478

    Article  CAS  PubMed  Google Scholar 

  • Azuma A, Kobayashi S, Yakushui H, Yamada M, Mitani, N, Sato A (2015) VvmybA1 genotype determines grape skin color. VITIS J Grapevine Res 46:154

    Google Scholar 

  • Ban Y, Honda C, Hatsuyama Y, Igarashi M, Bessho H, Moriguchi T (2007) Isolation and functional analysis of a MYB transcription factor gene that is a key regulator for the development of red coloration in apple skin. Plant Cell Physiol 48:958–970

    Article  CAS  PubMed  Google Scholar 

  • Benson CA, Kurz TL, Thackray VG (2013) A human FSHB promoter SNP associated with low FSH levels in men impairs LHX3 binding and basal FSHB transcription. Endocrinology 154:3016–3021

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Bowers JE, Dangl GS, Vignani R, Meredith CP (1996) Isolation and characterization of new polymorphic simple sequence repeat loci in grape (Vitis vinifera L.). Genome 39:628–633

    Article  CAS  PubMed  Google Scholar 

  • Burow MD, Chlan CA, Sen P, Murai N (1990) High frequency generation of transgenic tobacco plants after modified leaf disk cocultivation with Agrobacterium tumefaciens. Plant Mol Biol Rep 8:124–139

    Article  Google Scholar 

  • Castellarin SD, Gaspero GD (2007) Transcriptional control of anthocyanins biosynthetic genes in extreme phenotypes for berry pigmentation of naturally occurring grapevines. BMC Plant Biol 7:1

    Article  Google Scholar 

  • Castellarin SD, Gaspero GD, Marconi R, Nonis A, Peterlunger E, Paillard S, Testolin R (2006) Color variation in red grapevines (Vitis vinifera L.): genomic organization, expression of flavonoid 3′-hydroxylase, flavonoid 3′5′-hydroxylase genes and related metabolite profiling of red cyanidin-/blue delphinidin-based anthocyaninss in berry skin. BMC Genom 7:1

    Article  Google Scholar 

  • Du X, Wang Y, Guan C, Zeng M, Mo R, Xie F, Luo Z (2015) DKRE1-The first full-length Ty1-copia-like retrotransposon in persimmon: isolation, characteristic and potential involvement in occurrence of bud mutations. Sci Hortic Amsterdam 184:149–159

    Article  CAS  Google Scholar 

  • Espley RV, Hellens RP, Putterill J, Stevenson DE, Kutty-Amma S, Allan AC (2007) Red colouration in apple fruit is due to the activity of the MYB transcription factor, MdMYB10. Plant J 49:414–427

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Espley RV, Brendolise C, Chagné D, Kutty-Amma S, Green S, Volz R, Putterill J, Schouten HJ, Gardiner SE, Hellens RP, Allan AC (2009) Multiple repeats of a promoter segment causes transcription factor autoregulation in red apples. Plant Cell 21:168–183

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Fang J, Liu D, Ma Z (2002) Constructiong mango (Mangifera indica L.) genetic map using markers for double heterozygous loci. Fen zi zhi wu yu zhong 1:313–319

    Google Scholar 

  • Han YJ, Kim YM, Hwang OJ, Kim JI (2015) Characterization of a small constitutive promoter from Arabidopsis translationally controlled tumor protein (AtTCTP) gene for plant transformation. Plant Cell Rep 34:265–275

    Article  CAS  PubMed  Google Scholar 

  • Holton TA, Cornish EC (1997) Genetics and biochemistry of anthocyanins biosynthesis. Plant Cell 7:1071–1083

    Article  Google Scholar 

  • Kobayashi S, Ishimaru M, Hiraoka K, Honda C (2002) Myb-related genes of the Kyoho grape (Vitis labruscana) regulate anthocyanins biosynthesis. Planta 215:924–933

    Article  CAS  PubMed  Google Scholar 

  • Kobayashi S, Goto-Yamamoto N, Hirochika H (2004) Retrotransposon-induced mutations in grape skin color. Science 304:982–982

    Article  PubMed  Google Scholar 

  • Kobayashi S, Goto-Yamamoto N, Hirochika H (2005) Association of VvmybA1 gene expression with anthocyanins production in grape (Vitis vinifera) skin-color mutants. Soc Hort Sci 74:196–203

    Article  CAS  Google Scholar 

  • Lamboy WF, Alpha CG (1998) Using simple sequence repeats (SSRs) for DNA fingerprinting germplasm accessions of grape (Vitis L.) species. J Am Soc Hort Sci 123:182–188

    CAS  Google Scholar 

  • Li YC, Meng XJ, Qi Z, Yu N (2008) Essential substance content of different blueberry and the effect of treatment on the blueberry anthocyanins. Sci Technol Food Ind 5:048

    Google Scholar 

  • Li ZT, Dhekney SA, Gray DJ (2011) Use of the VvMybA1 gene for non-destructive quantification of promoter activity via color histogram analysis in grapevine (Vitis vinifera) and tobacco. Transgenic Res 20:1087–1097

    Article  CAS  PubMed  Google Scholar 

  • Li H, Yang X, Zhu L, Yi H, Chai L, Deng X (2015) Parentage analysis of natural citrus hybrid ‘Zhelong Zhoupigan’ based on nuclear and chloroplast SSR markers. Sci Hortic Amsterdam 186:24–30

    Article  CAS  Google Scholar 

  • Lijavetzky D, Ruiz-García L, Cabezas JA, De Andrés MT, Bravo G, Ibáñez A, Martínez-Zapater JM (2006) Molecular genetics of berry colour variation in table grape. Mol Genet Genomics 276:427–435

    Article  CAS  PubMed  Google Scholar 

  • Mazza G, Francis FJ (1995) Anthocyanins in grapes and grape products. Crit Rev Food Sci Nutr 35:341–371

    Article  CAS  PubMed  Google Scholar 

  • Palapol Y, Ketsa S, Wang KL, Ferguson IB, Allan AC (2009) A MYB transcription factor regulates anthocyanins biosynthesis in mangosteen (Garcinia mangostana L.) fruit during ripening. Planta 229:1323–1334

    Article  CAS  PubMed  Google Scholar 

  • Richard VE, Hellens RP, Putterill J, Stevenson DE, Kutty-Amma S, Allan AC (2007) Red colouration in apple fruit is due to the activity of the MYB transcription factor, MdMYB10. Plant J 49:414–427

    Article  Google Scholar 

  • Rinaldo A, Cavallini E, Jia Y, Moss SM, McDavid DA, Hooper LC, Walker AR (2015) A grapevine anthocyanin acyltransferase, transcriptionally regulated by VvMYBA, can produce most acylated anthocyanins present in grape skins. Plant physiol 169:1897–1916

    CAS  PubMed  PubMed Central  Google Scholar 

  • Sefc KM, Regner F, Turetschek E, Glossl J, Steinkellner H (1999) Identification of microsatellite sequences in Vitis riparia and their applicability for genotyping of different Vitis species. Genome 42:1–7

    Article  Google Scholar 

  • Shen Q, Zhang P, Ho TH (1996) Modular nature of abscisic acid (ABA) response complexes: composite promoter units that are necessary and sufficient for ABA induction of gene expression in barley. Plant Cell 8:1107–1119

  • Sousa JSI (1996) Uvas para o Brasil. FEALQ 1, Piracicaba

    Google Scholar 

  • Takos AM, Jaffe FW, Jacob SR, Bogs J, Robinson SP, Walker AR (2006) Light-induced expression of a MYB gene regulates anthocyanins biosynthesis in red apples. Plant Physiol 142:1216–1232

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Thomas MR, Scott NS (1993) Microsatellite repeats in grapevine reveal DNA polymorphisms when analyzed as sequence-tagged sites (STSs). Theor Appl Genet 86:985–990

    Article  CAS  PubMed  Google Scholar 

  • Walker AR, Lee E, Robinson SP (2006) Two new grape cultivars, bud sports of Cabernet Sauvignon bearing pale-colored berries, are the result of deletion of two regulatory genes of the berry color locus. Plant Mol Biol 62:623–635

    Article  CAS  PubMed  Google Scholar 

  • Walker AR, Lee E, Bogs J, McDavid DA, Thomas MR, Robinson SP (2007) White grapes arose through the mutation of two similar and adjacent regulatory genes. Plant J 49:772–785

    Article  CAS  PubMed  Google Scholar 

  • Wang XW, Xiong AS, Yao QH, Zhang Z, Qiao YS (2010) Direct isolation of high-quality low molecular weight RNA of pear peel from the extraction mixture containing nucleic acid. Mol Biotechnol 44:61–65

    Article  CAS  PubMed  Google Scholar 

  • Yakushiji H, Kobayashi S, Goto-Yamamoto N, Tae Jeong S, Sueta T, Mitani N, Azuma A (2006) A skin color mutation of grapevine, from black-skinned Pinot Noir to green-skinned Pinot Blanc, is caused by deletion of the functional VvmybA1 allele. Biosci Biotech Bioch 70:1506–1508

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Jianmin Tao.

Electronic supplementary material

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Xu, Y., Jiang, N., Zhang, Y. et al. A SNP in the promoter region of theVvmybA1 gene is responsible for differences in grape berry color between two related bud sports of grape. Plant Growth Regul 82, 457–465 (2017). https://doi.org/10.1007/s10725-017-0272-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10725-017-0272-5

Keywords

Navigation