Abstract
Cultivating insect pest-resistant varieties is one of the most effective ways to prevent or mitigate pest infestation in Chinese cabbage (Brassica campestris ssp. chinensis). Via the agrobacterium tumefaciens-mediated transformation method, this study introduced the protease inhibitor encoding gene sporamin into two widely cultured cultivars ‘Youdonger’ and ‘Shanghaiqing’, of the common variety of Chinese cabbages (B. campestriss ssp. chinensis var. communis), getting transgenic plants with high sporamin expression. In vitro insect bioassays indicated that, compared with the wild type plants, the transgenic plants exhibited improved resistance to diamondback moth (Plutella xylostella L.) The analysis of inheritance pattern of exogenous sporamin in the progenies of single copy insertion transgenic lines demonstrated that sporamin could be inherited and expressed stably in transgenic progenies. Field survey of the insect resistance under the normal culture condition confirmed the enhanced resistance in transgenic progenies to diamondback moth. Our results strongly suggest that sporamin is an efficient candidate gene for insect-resistant genetic engineering in Chinese cabbage.
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Acknowledgements
This work was supported by the Key Technology Innovation Team of Zhejiang Province (No. 2013TD05), the Sci-Technology Project of Zhejiang Province (2009C32026), and the Grand Science and Technology Special Project of Zhejiang Province (No. 2012C12903). The authors would like to thank Dr. Kai-Wun Yeh (Taiwan University) and Dr. Xuexin Cheng (Zhejiang University) for providing the vector and diamondback moth larvae, respectively.
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Cui, J., Li, M., Qiu, L. et al. Stable expression of exogenous imported sporamin in transgenic Chinese cabbage enhances resistance against insects. Plant Growth Regul 81, 543–552 (2017). https://doi.org/10.1007/s10725-016-0231-6
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DOI: https://doi.org/10.1007/s10725-016-0231-6