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The L-type lectin receptor-like kinase (PnLecRLK1) from the Antarctic moss Pohlia nutans enhances chilling-stress tolerance and abscisic acid sensitivity in Arabidopsis

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Abstract

Lectin receptor-like kinases (LecRLK) are widespread in higher plants and their effects on abiotic stress tolerance are gradually being reported. However, little information is available on LecRLK functions in bryophytes. Here, an L-type LecRLK gene (PnLecRLK1) was characterized from the Antarctic moss Pohlia nutans. Subcellular localization analysis revealed that PnLecRLK1 was a plasma membrane protein. The expression of PnLecRLK1 was rapidly induced by simulated cold, salt, and drought stresses as well as by exogenously applied abscisic acid (ABA) and methyl jasmonate. Transgenic Arabidopsis plants of overexpressing PnLecRLK1 exhibited enhanced tolerance to chilling-stress and increased ABA sensitivity. Additionally, the expression levels of genes in the C-repeat binding factor (CBF) signaling pathway such as AtCBF1, AtCBF2, AtCBF3 and AtCOR47 were markedly increased in transgenic Arabidopsis. Furthermore, the expression levels of ABA-responsive genes, such as AtABI4, AtABI5, AtMYB2 and AtDREB2A, were also significantly up-regulated in transgenic Arabidopsis. Therefore, our results suggested that PnLecRLK1 functions as a membrane-bound regulator that increases chilling stress tolerance and ABA sensitivity to enable P. nutans to adapt to polar climates.

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Acknowledgments

This work was supported by National Natural Science Foundation of China (41206176 and 41476174), Basic Scientific Fund for National Public Research Institutes (2014T04), Natural Science Foundation of Shandong Province (ZR2014DQ012) and Construction of the marine germplasm resource library (12PYY001SF08-HYYS-1).

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  1. Key Laboratory of Marine Bioactive Substance, Marine Ecology Research Center, The First Institute of Oceanography, State Oceanic Administration, 6 Xian-Xia Ling Road, Laoshan District, Qingdao, China

    Shenghao Liu & Zhaohui Zhang

  2. National Glycoengineering Research Center, School of Life Sciences, Shandong University, 27 Shanda South Road, Licheng District, Jinan, China

    Jing Wang, Kaoshan Chen & Pengying Zhang

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  1. Shenghao Liu
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  4. Zhaohui Zhang
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Correspondence to Zhaohui Zhang or Pengying Zhang.

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Liu, S., Wang, J., Chen, K. et al. The L-type lectin receptor-like kinase (PnLecRLK1) from the Antarctic moss Pohlia nutans enhances chilling-stress tolerance and abscisic acid sensitivity in Arabidopsis . Plant Growth Regul 81, 409–418 (2017). https://doi.org/10.1007/s10725-016-0217-4

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