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Analysis of physiobiochemical attributes, some key antioxidants and esculin content through HPLC in in vitro grown Cichorium intybus L. treated with ethylmethane sulfonate

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Abstract

This work covers studies on the effects of different concentrations of EMS (ES0, ES1, ES2, ES3, ES4, and ES5) on biochemical attributes, antioxidant enzymes and esculin content in in vitro grown Chicorium intybus. The bioactive compound esculin in in vitro raised shoots of C. intybus was determined by using high performance liquid chromatography-HPLC. EMS was used to induce in vitro mutagenesis for increase in the production of esculin content. The proline and lipid peroxidation increases with an increase in the concentration of EMS. The soluble protein, sugar content, antioxidant enzymes: superoxide dismutase, ascorbate peroxidase and glutathione reductase increase at lower concentrations of EMS and decrease at higher concentrations. EMS mutagenesis was found to be effective method for inducing increase in secondary metabolite production in C. intybus.

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Abbreviations

APX:

Ascorbate peroxidase

EMS:

Ethyl methanosulphate

GR:

Gluthioninereductase

HPLC:

High performance liquid chromatography

SOD:

Superoxide dismutase

TDZ:

Thiodiazuran

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Acknowledgments

Authors are highly thankful to Indian Institute of Integrative Medicine (IIIM) Srinagar, Kashmir for their help during experimental work.

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Authors and Affiliations

  1. Department of Botany, Faculty of Science, Jamia Hamdard, New Delhi, India

    Rehana Hamid &  Mahmooduzzafar

  2. Department of Environmental Science, Centre of Research for Development, University of Kashmir, Srinagar, India

    Azra N. Kamili

  3. Centre for Environmental Research, Near East University, Lefkosha, Turkish Republic of the Northern Cyprus

    Salih Gücel

  4. Botany Department, Science Faculty, Ege University, Bornova-Izmir, Turkey

    Münir Öztürk

  5. Department of Botany, S.P. College, Srinagar, 190 001, Jammu and Kashmir, India

    Parvaiz Ahmad

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  1. Rehana Hamid
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Hamid, R., Kamili, A.N., Mahmooduzzafar et al. Analysis of physiobiochemical attributes, some key antioxidants and esculin content through HPLC in in vitro grown Cichorium intybus L. treated with ethylmethane sulfonate. Plant Growth Regul 76, 233–241 (2015). https://doi.org/10.1007/s10725-014-9992-y

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  • DOI: https://doi.org/10.1007/s10725-014-9992-y

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