Abstract
Suppression subtractive hybridization method was used to profile gene changes in the latex between self-rooting juvenile clones (JCs) and donor clones (DCs) to better understand the molecular mechanism underlying the differences between these clones. 176 differentially expressed genes were identified, 95 of which were upregulated and 81 with reduced self-rooting JCs. These genes were classified as stress/defense response, rubber biosynthesis, metabolism and energy, protein metabolism, signal transduction transcription, and post-transcription. Systematic analyses of the differentially expressed genes between self-rooting JCs and DCs suggest that rubber biosynthesis, production, and scavenging of reactive oxygen species may have significant functions in high-yielding self-rooting JCs. This result contributes to better understanding on the molecular mechanism underlying the difference between self-rooting JCs and DCs.
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Abbreviations
- DCs:
-
Donor clones
- HMGR-CoA:
-
3-Hydroxy-3-methylglutaryl coenzyme A reductase
- JCs:
-
Juvenile clones
- ROS:
-
Reactive oxygen species
- REF:
-
Rubber elongation factor
- SRPP:
-
Small rubber particle protein
- SSH:
-
Suppression subtractive hybridization
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Acknowledgments
This work was supported by the National Natural Science Foundation of China (Nos. 31170634), Hainan Sciences and Technology Major Projects (ZDZX2013023-1) and a Chinese National Nonprofit Institute Research Grant of CATAS-ITBB (ITBB110205). We deeply thank Professor Xiong-Ting Chen (Institute of Tropical Biosciences and Biotechnology, CATAS) for his collaboration in treatment of plant materials.
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Li, HL., Guo, D. & Peng, SQ. Differential gene expression profiles in latex from Hevea brasiliensis between self-rooting juvenile and donor clones. Plant Growth Regul 74, 65–71 (2014). https://doi.org/10.1007/s10725-014-9900-5
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DOI: https://doi.org/10.1007/s10725-014-9900-5