Plant Growth Regulation

, Volume 68, Issue 2, pp 293–301

Effect of plant growth regulators on plant regeneration of Dioscorea remotiflora (Kunth) through nodal explants

  • A. Bernabé-Antonio
  • F. Santacruz-Ruvalcaba
  • F. Cruz-Sosa
Original paper

DOI: 10.1007/s10725-012-9717-z

Cite this article as:
Bernabé-Antonio, A., Santacruz-Ruvalcaba, F. & Cruz-Sosa, F. Plant Growth Regul (2012) 68: 293. doi:10.1007/s10725-012-9717-z

Abstract

Dioscorea remotiflora (Kunth) is an important wild plant that produces tuberous roots used as a source of food in the Western part of Mexico. Lack of planting material and inefficiency of traditional methods of propagation are the main constraints for implementing large-scale cultivation. In contrast, tissue culture techniques allow increasing multiplication and rapid production of plant material. In this regard, leaves or nodal segments were incubated on MS, B5 and WPM culture media with different PGRs in order to obtain an efficient micropropagation protocol. Leaves explants were unable to inducing shoots or callus. However, nodal segments produced axillary shoots and/or callus in all culture media. MS containing 2.33 μM KIN was the most suitable to inducing shoots; an average of 6.6 shoots per segment for 100 % explants was obtained, which displayed also the greater number of nodes (5.0) and leaves (7.9) per segment. A decrease on shoot proliferation was observed combining BA or KIN with 2,4-D or NAA. However, small brownish callus were induced on 100 % of segments using 2.33 μM KIN with 5.37 μM 2,4-D or 9.30 μM KIN plus 2.69 μM NAA. In contrast, by adding 2.69 μM NAA, 66.4 % of the nodal segments formed shoots and produced also yellowish friable callus on the base of the shoots. Shoots were easily rooted with 8.28 μM IBA (96.9 %), displaying the greatest root and shoot biomass, but maximum number of tuberous roots, and root or tuberous root biomass was produced increasing IBA (20.7 μM).

Keywords

Tissue cultureCulture mediaMicropropagationDry weightTuberous roots

Abbreviations

ANOVA

Analysis of variance

BA

N6-Benzyladenine

DW

Dry weight

IBA

Indole-3-butyric acid

PGRs

Plant growth regulators

PVP

Polyvinylpyrrolidone

KIN

Kinetin

NAA

α-Naphthaleneacetic acid

WPM

Woody plant medium

2,4-D

2,4-Dichlorophenoxyacetic acid

Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • A. Bernabé-Antonio
    • 1
  • F. Santacruz-Ruvalcaba
    • 1
  • F. Cruz-Sosa
    • 2
  1. 1.Department of Agricultural ProductionUniversity Center of Biological and Agricultural Sciences, University of GuadalajaraZapopanMexico
  2. 2.Department of Biotechnology, Division of Biological and Health SciencesMetropolitan Autonomous University-Iztapalapa CampusMexico D.FMexico