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The dynamics of nutrient utilization and growth of apple root stock ‘M9 EMLA’ in temporary versus continuous immersion bioreactors

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Abstract

The present study investigated the dynamics of nutrient utilization and various growth and physiological parameters during in vitro proliferation of apple root stock ‘M9 EMLA’ in two different bioreactor systems, i.e. temporary and continuous immersions. Individual shoots obtained from temporary immersion system had higher dry mass and were of better quality than those obtained from continuous immersion. In continuous immersion bioreactor, apple shoots appeared to utilize more nutrients from liquid culture medium than that from temporary immersion. The shoot growth was limited by the availability of phosphate and nitrogen in continuous immersion system. The shoots produced in temporary immersion bioreactor showed higher photosynthetic rate, maximum quantum yield of photosystem-II and slow but steady rate of nutrient absorption, indicating the occurrence of higher photomixotrophic metabolism. The study also showed that high level of antioxidant scavenging enzymes in shoots grown in continuous immersion system induced physiological changes to foster adaptation to stresses.

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Abbreviations

APX:

Ascorbate peroxidase

BTBB:

Balloon-type bubble bioreactor

BA:

N6-benzyladenine

CAT:

Catalase

IBA:

Indole-3-butyric acid

G-POD:

Guaiacol peroxidase

GA3 :

Gibberellic acid

GR:

Glutathione reductase

MS:

Murashige and Skoog’s (1962) medium

PPFD:

Photosynthetic photon flux density

ROS:

Reactive oxygen species

SOD:

Superoxide dismutase

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Acknowledgements

This work was financially supported by the Ministry of education and Human Resource Development (MOE), the Ministry of Commerce, Industry and Energy (MOCIE) and the Ministry of Labor (MOLAB) through the fostering project of the Lab of Excellency. DC acknowledges the KOSEF for providing financial assistance in the form of “Long-term Foreign Scientist Program”. SKD also acknowledges the financial assistance from INSA-KOSEF Exchange Program.

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Authors and Affiliations

  1. Floriculture Section, National Botanical Research Institute, Lucknow, 226001, India

    D. Chakrabarty & S. K. Datta

  2. Department of Horticulture, Faculty of Agriculture, Kafr El Sheikh University, Kafr El Sheikh, 33516, Egypt

    Y. H. Dewir

  3. Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University, Cheong-ju, 361-763, Korea

    D. Chakrabarty, Y. H. Dewir, E. J. Hahn & K. Y. Paek

Authors
  1. D. Chakrabarty
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  2. Y. H. Dewir
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  3. E. J. Hahn
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  4. S. K. Datta
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  5. K. Y. Paek
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Correspondence to K. Y. Paek.

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Chakrabarty, D., Dewir, Y.H., Hahn, E.J. et al. The dynamics of nutrient utilization and growth of apple root stock ‘M9 EMLA’ in temporary versus continuous immersion bioreactors. Plant Growth Regul 51, 11–19 (2007). https://doi.org/10.1007/s10725-006-9115-5

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  • DOI: https://doi.org/10.1007/s10725-006-9115-5

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