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Phylogenetic relationship of 40 species of genus Aloe L. and the origin of an allodiploid species revealed by nucleotide sequence variation in chloroplast intergenic space and cytogenetic in situ hybridization

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Abstract

Aloe species, which have been used as medicinal plants, belong to the Asphodelaceae family consisting of 530 species. In this study, genetic diversity and phylogenetic relationships among 40 Aloe species including a putative interspecies hybrid were analyzed using PCR band profiles from eight chloroplast intergenic space markers and nucleotide sequence diversity in the psbK–psbI intergenic region. A phylogenetic tree based on psbK–psbI sequences supported the revised classification of the genus Aloe as polyphyletic with several species be re-allocated into three genera Kumara, Aloidendron, and Aloiampelos. Further, the origin of the putative interspecies Aloe hybrid was characterized through molecular cytogenetics. Fluorescence and genomic in situ hybridization illustrated that the hybrid has a bimodal karyotype with a chromosome complement of 2n = 14, of which complementary halves were derived from two parental species, A. vera and A. arborescens. These findings revealed that the hybrid species was allodiploid. The phylogenetic analysis showed that A. arborescens was the maternal genome donor of the hybrid, as both have identical chloroplast genome sequences. We thus conclude that the allodiploid hybrid should be called A. arborescens × A.vera.

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References

  • Adams SP, Leitch IJ, Bennett MD, Chase MW, Leitch AR (2000) Ribosomal DNA evolution and phylogeny in Aloe (Asphodelaceae). Am J Bot 87:1578–1583

    Article  PubMed  CAS  Google Scholar 

  • Baldoni L, Guerrero C, Sossey-Aloui K, Abbott A, Angiolillo A, Lumaret R (2002) Phylogenetic relationships among Olea species, based on nucleotide variation at a non-coding chloroplast DNA region. Plant Biol 4:346–351

    Article  CAS  Google Scholar 

  • Brandham P, Cutler D (1978) Influence of chromosome variation on the organisation of the leaf epidermis in a hybrid Aloe (Liliaceae). Bot J Linn Soc 77:1–16

    Article  Google Scholar 

  • Daru BH, Manning JC, Boatwright JS, Maurin O, Maclean N, Schaefer H, Kuzmina M, van der Bank M (2013) Molecular and morphological analysis of subfamily Alooideae (Asphodelaceae) and the inclusion of Chortolirion in Aloe. Taxon 62:62–76

    Google Scholar 

  • Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19:11–15

    Google Scholar 

  • Fauré S, Noyer J-L, Carreel F, Horry J-P, Bakry F, Lanaud C (1994) Maternal inheritance of chloroplast genome and paternal inheritance of mitochondrial genome in bananas (Musa acuminata). Curr Genet 25:265–269

    Article  PubMed  Google Scholar 

  • Fentaw E, Dagne K, Rønsted N, Demissew S, Grace OM (2013) Karyotypes in Ethiopian Aloe species (Xanthorrhoeaceae: Asphodeloideae). Kew Bull 68:599–607

    Article  Google Scholar 

  • Grace OM, Klopper RR, Smith GF, Crouch NR, Figueiredo E, Ronsted N, Van Wykae (2013) A revised generic classification for Aloe (Xanthorrhoeaceae subfam. Asphodeloideae). Phytotaxa 76:7–14

  • Grindlay D, Reynolds T (1986) The Aloe vera phenomenon: a review of the properties and modern uses of the leaf parenchyma gel. J Ethnopharmacol 16:117–151

    Article  PubMed  CAS  Google Scholar 

  • Gustafson P, Czernicka M, Mscichowska A, Klein M, Muras P, Grzebelus E (2010) Paternity determination of interspecific rhododendron hybrids by genomic in situ hybridization (GISH). Genome 53:277–284

    Article  CAS  Google Scholar 

  • Hao DC, Huang B, Yang L (2008) Phylogenetic relationships of the genus Taxus inferred from chloroplast intergenic spacer and nuclear coding DNA. Biol Pharm Bull 31:260–265

    Article  CAS  Google Scholar 

  • Harpke D, Peterson A, Hoffmann MH, Röser M (2006) Phylogenetic evaluation of chloroplast trnL–trnF DNA sequence variation in the genus Mammillaria (Cactaceae). Schlechtendalia 14:7–16

    Google Scholar 

  • Imery-Buiza J, Raymúndez MB, Menéndez-Yuffa A (2008) Karyotypic variability in experimental diploid and triploid hybrids of Aloe vera  × A. saponaria. Cytologia 73:305–311

    Article  Google Scholar 

  • Kim JH, Jung J-Y, Choi H-I, Kim N-H, Park JY, Lee Y, Yang T-J (2013) Diversity and evolution of major Panax species revealed by scanning the entire chloroplast intergenic spacer sequences. Genet Resour Crop Evol 60:413–425

    Article  Google Scholar 

  • Klopper RR, Van Wyk AE, Smith GF (2010) Phylogenetic relationships in the family Asphodelaceae (Asparagales). Biodivers Ecol 3:9–36

    Google Scholar 

  • Lee YS, Yang TJ, Park SU, Baek JH, Wu S, Lim KB (2011) Induction and proliferation of adventitious roots from Aloe vera leaf tissues for in vitro production of aloe-emodin. Plant Omics 4:190–194

    CAS  Google Scholar 

  • Marasek A, Hasterok R, Wiejacha K, Orlikowska T (2004) Determination by GISH and FISH of hybrid status in Lilium. Hereditas 140:1–7

    Article  PubMed  Google Scholar 

  • Markova M, Lengerova M, Zluvova J, Janousek B, Vyskot B (2006) Karyological analysis of an interspecific hybrid between the dioecious Silene latifolia and the hermaphroditic Silene viscosa. Genome 49:373–379

    Article  PubMed  Google Scholar 

  • Palmer JD (1985) Comparative organization of chloroplast genomes. Annu Rev Genet 19:325–354

    Article  PubMed  CAS  Google Scholar 

  • Park YI, Lee SK (2006) New perspectives on Aloe. In: Sung CK (ed) The history of Aloe. Springer, Berlin, pp 7–17

    Google Scholar 

  • Rieger R, Michaelis A, Green MM (1991) Glossary of genetics, 5th edn. Springer, Berlin

    Book  Google Scholar 

  • Smith GF, Klopper RR, Crouch NR (2008) Aloe arborescens (Asphodelaceae: Alooideae) and CITES. Haseltonia 14:189–198

    Article  Google Scholar 

  • Smith GF, Klopper RR, Figueiredo E, Crouch NR (2012) Aspects of the taxonomy of Aloe arborescens Mill. (Asphodelaceae: Alooideae). Bradleya 30:127–137

    Google Scholar 

  • Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Treutlein J, Smith G, Wyk BE, Wink M (2003a) Evidence for the polyphyly of Haworthia (Asphodelaceae subfamily Alooideae; Asparagales) inferred from nucleotide sequences of rbcL, matK, ITS1 and genomic fingerprinting with ISSR-PCR. Plant Biol 5:513–521

    Article  CAS  Google Scholar 

  • Treutlein J, Smith GF, Van Wyk B-E, Wink M (2003b) Phylogenetic relationships in Asphodelaceae (subfamily Alooideae) inferred from chloroplast DNA sequences (rbcL, matK) and from genomic fingerprinting (ISSR). Taxon 52:193–207

    Article  Google Scholar 

  • Vences M, Wollenberg KC, Vieites DR, Lees DC (2009) Madagascar as a model region of species diversification. Trends Ecol Evol 24:456–465

    Article  PubMed  Google Scholar 

  • Waminal NE, Kim HH (2012) Dual-color FISH karyotype and rDNA distribution analyses on four Cucurbitaceae species. Hortic Environ Biotechnol 53:49–56

    Article  Google Scholar 

  • Yoo KO, Jang SK (2010) Infrageneric relationships of Korean Viola based on eight chloroplast markers. J Syst Evol 48:474–481

    Article  Google Scholar 

Download references

Acknowledgments

This study was supported by the Next-Generation BioGreen 21 Program (No. PJ011030-01) of the Rural Development Administration and Kim Jeong Moon Aloe Co. Republic of Korea.

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Authors and Affiliations

  1. Department of Plant Science, Plant Genomics and Breeding Institute, and Research Institute of Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University, Seoul, 151-921, Korea

    Yun Sun Lee, Hye Mi Park, Nam-Hoon Kim, Nomar E. Waminal, Yeon Jeong Kim & Tae-Jin Yang

  2. Department of Horticultural Science, Kyungpook National University, Daegu, 702-701, Korea

    Ki-Byung Lim

  3. Kim Jeong Moon Aloe Co. LTD, 3rd Fl., BaekJae Bldg., 1580-10 SeoCho3-Dong, SeoCho-Gu, Seoul, 137-875, Korea

    Jin Hong Baek

  4. Department of Life Science, Plant Biotechnology Institute, Sahmyook University, Seoul, 139-742, Korea

    Hyun Hee Kim

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  1. Yun Sun Lee
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  2. Hye Mi Park
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  3. Nam-Hoon Kim
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  4. Nomar E. Waminal
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  8. Hyun Hee Kim
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  9. Tae-Jin Yang
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Correspondence to Tae-Jin Yang.

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Lee, Y.S., Park, H.M., Kim, NH. et al. Phylogenetic relationship of 40 species of genus Aloe L. and the origin of an allodiploid species revealed by nucleotide sequence variation in chloroplast intergenic space and cytogenetic in situ hybridization. Genet Resour Crop Evol 63, 235–242 (2016). https://doi.org/10.1007/s10722-015-0243-5

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  • DOI: https://doi.org/10.1007/s10722-015-0243-5

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