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Centromeric position and genomic allocation of a repetitive sequence isolated from chromosome 18D of hexaploid oat, Avena sativa L.

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Abstract

Cultivated oat (Avena sativa L.) is allohexaploid and contains three genomes (A, C, and D). By using fluorescence in situ hybridization, a 391 bp repetitive DNA fragment (‘A336’) isolated from oat chromosome 18D was predominantly localized in centromeric regions of mitotic metaphase chromosomes of oat accession ‘CN64226’. Assays of simultaneous and sequential co-hybridizations with the C genome-specific repetitive DNA probe ‘pAm1’ isolated from tetraploid A. murphyi Ladiz. (AACC) and the application of different concentrations of the A336 probe revealed that the A336 DNA segment is more abundant in chromosomes of the A- and D-genomes than in chromosomes belonging to the C-genome. Our results provide information which may be useful in future cytogenetic studies and in aiding physical genome assembly.

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Acknowledgments

This work was financially supported by the Science and Technology Bureau of Sichuan Province, China.

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Authors and Affiliations

  1. Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, 611130, Sichuan, China

    Xiaomei Luo, Haiqin Zhang, Houyang Kang, Xing Fan, Yi Wang, Lina Sha & Yonghong Zhou

  2. Eastern Cereal and Oilseed Research Centre, Agriculture and Agri-Food Canada, KW Neatby Bldg., Central Experimental Farm, 960 Carling Avenue, Ottawa, ON, K1A 0C6, Canada

    Xiaomei Luo, Nicholas A. Tinker & Charlene P. Wight

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  1. Xiaomei Luo
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  2. Nicholas A. Tinker
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  9. Yonghong Zhou
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Correspondence to Yonghong Zhou.

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Luo, X., Tinker, N.A., Zhang, H. et al. Centromeric position and genomic allocation of a repetitive sequence isolated from chromosome 18D of hexaploid oat, Avena sativa L.. Genet Resour Crop Evol 62, 1–4 (2015). https://doi.org/10.1007/s10722-014-0170-x

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  • DOI: https://doi.org/10.1007/s10722-014-0170-x

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