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Phenotypic characterization of the Miami World Collection of sugarcane (Saccharum spp.) and related grasses for selecting a representative core

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Abstract

The Saccharum L. genus includes important crops that are utilized for sugar and fuel production. The World Collection of Sugarcane and Related Grasses (World Collection) in Miami, FL contains diverse and potentially useful germplasm for this and related genera; however, this collection has been underutilized because little is known about the traits of its accessions. Our objectives were to phenotypically characterize the World Collection and select a representative core collection that could then be studied intensively. In total, eight morphological traits of the World Collection were evaluated three times in 1 year. A core of 300 accessions that included each species in the World Collection was selected by using the Maximization Strategy in MStrat software. The core had a higher diversity rating than random selections of 300 accessions. The Shannon–Weaver Diversity Index scores of the core and whole collection were similar indicating that the majority of the diversity was captured by the core collection. The ranges and medians between the core and World Collection were similar; only two of the trait medians were not significant at P = 0.05 using the non-parametric Wilcoxon method and the coincidence rate (CR % = 96.2) was high (>80) indicating that extreme values were retained. Thus, the phenotypic diversity of these traits in the World Collection was well represented by the core collection. Agronomic studies on the core should be useful for characterizing the World Collection and genes for useful traits should be available in the core collection.

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Acknowledgments

This research was supported by the Office of Science (BER), U.S. Department of Energy. The authors wish to acknowledge the technical assistance of the following individuals in phenotypic evaluations: Miriam Baltazar, Velton Banks, Billy Cruz, Moiad Kanaan, Joseph Orsenigo, Matthew Paige, Leo Perez, Ken Peterkin, Kristen Polacik, Ricardo Ramirez, Jhonnie Tejeda, Juan Tejeda, and Liping Wang.

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Authors and Affiliations

  1. USDA-ARS Sugarcane Field Station, Canal Point, FL, 33438, USA

    James Todd, Barry Glaz, Sushma Sood & Jack C. Comstock

  2. Agronomy Department, Plant Molecular and Cellular Biology Program, Genetics Institute, University of Florida, Gainesville, FL, 32610, USA

    Jianping Wang

  3. USDA-ARS Subtropical Horticulture Research Station, Miami, FL, 33158, USA

    Tomas Ayala-Silva, Osman A. Gutierrez & David N. Kuhn

  4. Syngenta Seeds, Inc., Longmont, CO, 80501, USA

    Neil C. Glynn

  5. Sugar Crops Research Institute, Mardan, Khyber Pakhtunkhwa, Pakistan

    Mohammad Tahir

  6. Agronomy Department, Genetics Institute, University of Florida, Gainesville, FL, 32610, USA

    Spurthi N. Nayak

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  1. James Todd
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  2. Jianping Wang
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  3. Barry Glaz
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  10. Mohammad Tahir
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  11. Jack C. Comstock
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Correspondence to James Todd.

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Todd, J., Wang, J., Glaz, B. et al. Phenotypic characterization of the Miami World Collection of sugarcane (Saccharum spp.) and related grasses for selecting a representative core. Genet Resour Crop Evol 61, 1581–1596 (2014). https://doi.org/10.1007/s10722-014-0132-3

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