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Evaluation of genetic structure of Korean wild soybean (Glycine soja) based on saponin allele polymorphism

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Abstract

Korean wild soybeans (Glycine soja) hypocotyl exhibit seven common saponin allele combinations: Sg-1 a /sg-4/sg-6 (phenotype: Aa), Sg-1 b /sg-4/sg-6 (Ab), Sg-1 a /Sg-4/sg-6 (AaBc), Sg-1 b /Sg-4/sg-6 (AbBc), Sg-1 a /sg-4/Sg-6 (Aa + α), Sg-1 a /Sg-4/Sg-6 (AaBc + α) and Sg-1 b /Sg-4/Sg-6 (AbBc + α). Diversity of saponin allele frequency (Haf) and saponin allele combination frequency (Hacf) was evaluated by Shannon–Weaver Index that was ranged from 0.75 to 1.04 and 0.28 to 1.25, respectively. High values of Haf and Hacf were found in Jeollanam-do and its adjoining regions Jeollabuk-do and Gyeongsangnam-do. The genetic index of Korean wild soybean increased towards south from north and west from east regions. The frequency of Sg-1 b and Sg-6 allele combinations increased towards south region (Jeollanam-do). These results suggest that Jeollanam-do region is the primary center of diversity, Jeollabuk-do and Gyeongsangnam-do regions are the secondary center of diversity and the remaining regions (Gyeonggi-do, Gangwon-do, Chungcheongbuk-do, Chungcheongnam-do, Jeollabuk-do, Gyeongsangbuk-do and Jeju-do) are the tertiary center of diversity for soyasaponin polymorphism in Korea. Furthermore, saponin allele frequency distribution suggested that alleles Sg-1 b and Sg-6 dispersed from Jeollanam-do to other regions of Korea. Our findings have laid the foundation for uncovering genetic diversity in Korean wild soybeans that could be exploited in soybean breeding programs.

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Acknowledgments

We thank Dr. Sang-Mi Sun for maintaining the germplasm collection. This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2010-0013600).

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Authors and Affiliations

  1. Department of Biotechnology, Chonnam National University, Yeosu, 550-749, Chonnam, Korea

    Panneerselvam Krishnamurthy, Jae Min Lee & Gyuhwa Chung

  2. Department of Applied Biological Chemistry, Iwate University, Morioka, Iwate, 020-8550, Japan

    Chigen Tsukamoto & Yuya Takahashi

  3. USDA/ARS, Department of Crop Science, National Soybean Research Laboratory, University of Illinois, Urbana, IL, 61801, USA

    Ram J. Singh

  4. Division of Plant Biosciences, Kyungpook National University, Daegu, 702-701, Korea

    Jeong Dong Lee

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  1. Panneerselvam Krishnamurthy
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  2. Jae Min Lee
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  3. Chigen Tsukamoto
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  4. Yuya Takahashi
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  5. Ram J. Singh
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  7. Gyuhwa Chung
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Correspondence to Chigen Tsukamoto or Gyuhwa Chung.

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Krishnamurthy, P., Lee, J.M., Tsukamoto, C. et al. Evaluation of genetic structure of Korean wild soybean (Glycine soja) based on saponin allele polymorphism. Genet Resour Crop Evol 61, 1121–1130 (2014). https://doi.org/10.1007/s10722-014-0095-4

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  • DOI: https://doi.org/10.1007/s10722-014-0095-4

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