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Systemic activation of NF-κB driven luciferase activity in transgenic mice fed advanced glycation end products modified albumin

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Abstract

Advanced glycation end products (AGEs) are stable end products of the Maillard reaction and accumulate with progressing ageing and degenerative diseases. Significant amounts of AGE-modified peptides are also consumed with processed food. AGEs bind to specific receptors, especially the receptor of AGEs (RAGE). Activation of RAGE then evokes intracellular signalling, finally resulting in the activation of the NF-κB transcription factor and therefore a proinflammatory state. We here analysed, whether NF-κB is activated in short term upon feeding an AGE-modified protein in-vivo. Transgenic mice expressing firefly luciferase under the control of an NF-κB responsive promoter were intraperitoneally injected or fed with AGE-modified- or control albumin and luciferase expression was analysed by in-vivo imaging and by in-vitro by determination of luciferase enzyme activity in heart, lung, gut, spleen, liver and kidney. In all organs, an activation of the luciferase reporter gene was observed in response to AGE-BSA feeding, however with different intensity and timing. The gut exhibited highest luciferase activity and this activity peaked 6–8 h post AGE-feeding. In heart and kidney, luciferase activity increased for up to 12 h post feeding. All other organs tested, exhibited highest activity at 10 h after AGE-consumption. Altogether, these data demonstrate that feeding AGE-modified protein resulted in a transient and systemic activation of the NF-κB reporter.

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Acknowledgements

This study was in part funded by the Deutsche Forschungsgemeinschaft (DFG, Si-1317/1-1).

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Author notes

  1. Norbert Nass

    Present address: Institute of Pathology, Otto von Guericke University Magdeburg, Leipziger Str. 44, D- 39120, Magdeburg, Germany

Authors and Affiliations

  1. Department of Cardiac Surgery, Middle German Heart Centre, University Hospital Halle (Saale), Ernst Grube Str. 40, D-06120, Halle Saale, Germany

    Norbert Nass, Kristina Bayreuther & Andreas Simm

Authors
  1. Norbert Nass
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  2. Kristina Bayreuther
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  3. Andreas Simm
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Correspondence to Norbert Nass.

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All procedures performed in studies involving animals were in accordance with the ethical standards of the institution or practice at which the studies were conducted..

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Nass, N., Bayreuther, K. & Simm, A. Systemic activation of NF-κB driven luciferase activity in transgenic mice fed advanced glycation end products modified albumin. Glycoconj J 34, 157–161 (2017). https://doi.org/10.1007/s10719-017-9762-y

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  • DOI: https://doi.org/10.1007/s10719-017-9762-y

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