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Immuno-PCR technology for detection of natural human antibodies against Lec disaccharide

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Abstract

The development of an immuno-PCR assay for quantitation of low amounts of anti-glycan human antibodies is described. The sensitivity of the assay for determination of low-affinity anti-LeC IgM has been found to be 4 ng/ml (~100 pg per sample), thus being two orders of magnitude higher compared to the conventional ELISA with the same antigen.

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Acknowledgements

We are grateful to N.N. Tupitsyn and L.N. Liubchenko (Blokhin Cancer Research Center, Ministry of Health of the Russian Federation) for kindly providing us with serum samples.

The part of this study involving iPCR and the corresponding data analysis was supported by the Russian Science Foundation project no. 16-14-00136 (D. Yu. Ryazantsev, D.V. Voronina, and S.K. Zavriev) and the Grant of the President of the Russian Federation no. 4413.2015.4 (D. Yu. Ryazantsev). The immunological part of the study (K. Dobrochaeva, O. Galanina, and N. Bovin) was supported by the Russian Science Foundation project no. 14-50-00131.

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Authors and Affiliations

  1. JSC NPF DNA-Technology, Moscow, Russia

    Artem V. Maerle

  2. Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia

    Daria V. Voronina, Kira L. Dobrochaeva, Oxana E. Galanina, Nicolai V. Bovin, Sergei K. Zavriev & Dmitriy Yu Ryazantsev

  3. NRC Institute of Immunology, Federal Biomedical Agency of Russia, Moscow, Russia

    Leonid P. Alekseev

Authors
  1. Artem V. Maerle
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  2. Daria V. Voronina
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  3. Kira L. Dobrochaeva
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  4. Oxana E. Galanina
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  5. Leonid P. Alekseev
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  6. Nicolai V. Bovin
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  7. Sergei K. Zavriev
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  8. Dmitriy Yu Ryazantsev
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Correspondence to Artem V. Maerle.

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Maerle, A.V., Voronina, D.V., Dobrochaeva, K.L. et al. Immuno-PCR technology for detection of natural human antibodies against Lec disaccharide. Glycoconj J 34, 199–205 (2017). https://doi.org/10.1007/s10719-016-9751-6

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  • DOI: https://doi.org/10.1007/s10719-016-9751-6

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