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Structure and antitumor activity of the extracellular polysaccharides from Aspergillus aculeatus via apoptosis and cell cycle arrest

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Abstract

Two extracellular polysaccharides, designated as WPA and WPB, were isolated from the fungus Aspergillus aculeatus using Q-Sepharose fast flow and Sephacryl S-300 column chromatography. WPA composed of mannose and galactose in a molar ratio of 3.9:1.0, and WPB mainly contained mannose. The molecular weight of WPA and WPB was about 28.1 kDa and 21.0 kDa, respectively. On the basis of methylation and NMR analysis, the possible main chain of WPA was [→5)-β-D-Galf-(1 → 2,6)-α-D-Manp(1→], and WPB was mainly [→2,6)-α-D-Manp(1→], both with [α-D-Manp(1 → 2)-α-D-Manp(1 → 2)-α-D-Manp(1→] substituted at C-2 of [→2,6)-α-D-Manp(1→]. Meanwhile, WPA displayed a stronger anti-proliferative effect than WPB on HeLa, MCF-7 and MGC-803 cells in vitro. WPA and WPB could arrest HeLa cells in G2/M phase and induce HeLa cells apoptosis. Thus, our study provides evidence that WPA and WPB may be taken as potential candidates for treating cervical carcinoma.

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Acknowledgments

This work was supported by financial support from Hebei University, Natural Science Foundation of Hebei province in China (B2015201069), Youth Fund Project of Hebei Education Department (QN2014131), and Bureau of Baoding city science and technology project (14ZF083).

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Authors and Affiliations

  1. Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, Baoding, 071002, China

    Hongyan Li, Yan Xu, Xiaoke Wang & Huajie Zhu

  2. College of Chemistry and Environment Science, Hebei University, Baoding, 071002, China

    Hongyan Li, Yan Xu & Xiaoke Wang

  3. College of Pharmaceutical Science, Hebei University, Baoding, 071002, China

    Huajie Zhu

  4. Biology Institute of Shandong Academy of Sciences/Key Laboratory for Biosensors of Shandong Province, Jinan, 250014, China

    Xin Liu

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  1. Hongyan Li
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Correspondence to Hongyan Li or Huajie Zhu.

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Electronic supplementary material

Supplementary Fig. S1

NMR spectra of WPA and WPB. (a) 1H-1H COSY spectrum, (b) 1H-13C HMQC spectrum, (c) 1H-1H NOESY spectrum and (d) 1H-13C HMBC spectrum. A: [→2)-α-D-Manp(1→], B: [→2)-α-D-Manp(1→], C: [→2,6)-α-D-Manp(1→], D: [α-D-Manp(1→], E: [→5)-β-D-Galf(1→] (PDF 313 kb)

Supplementary Fig. S2

Cell cycle synchronization of HeLa cells with or without 10 % FBS for 24 h. (PDF 27 kb)

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Li, H., Liu, X., Xu, Y. et al. Structure and antitumor activity of the extracellular polysaccharides from Aspergillus aculeatus via apoptosis and cell cycle arrest. Glycoconj J 33, 975–984 (2016). https://doi.org/10.1007/s10719-016-9717-8

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  • DOI: https://doi.org/10.1007/s10719-016-9717-8

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