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Metabolic glycoengineering bacteria for therapeutic, recombinant protein, and metabolite production applications

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Abstract

Metabolic glycoengineering is a specialization of metabolic engineering that focuses on using small molecule metabolites to manipulate biosynthetic pathways responsible for oligosaccharide and glycoconjugate production. As outlined in this article, this technique has blossomed in mammalian systems over the past three decades but has made only modest progress in prokaryotes. Nevertheless, a sufficient foundation now exists to support several important applications of metabolic glycoengineering in bacteria based on methods to preferentially direct metabolic intermediates into pathways involved in lipopolysaccharide, peptidoglycan, teichoic acid, or capsule polysaccharide production. An overview of current applications and future prospects for this technology are provided in this report.

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Acknowledgments

Funding was obtained from the National Institutes of Health, NCI grant R01CA112314.

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Authors and Affiliations

  1. Department of Biomedical Engineering and the Translational Tissue Engineering Center, The Johns Hopkins University, Baltimore, MD, USA

    Christopher T. Saeui, Esteban Urias, Lingshu Liu, Mohit P. Mathew & Kevin J. Yarema

  2. Translational Tissue Engineering Center, The Johns Hopkins University, 5029 Robert H. & Clarice Smith Building, 400 North Broadway, Baltimore, MD, 21231, USA

    Kevin J. Yarema

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Saeui, C.T., Urias, E., Liu, L. et al. Metabolic glycoengineering bacteria for therapeutic, recombinant protein, and metabolite production applications. Glycoconj J 32, 425–441 (2015). https://doi.org/10.1007/s10719-015-9583-9

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