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Altered expression and glycosylation of plasma proteins in rheumatoid arthritis

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Abstract

Altered glycosylation of plasma proteins has been directly implicated in the pathogenesis of rheumatoid arthritis (RA). The present study investigated the changes in the Concanavalin-A (Con-A)-bound plasma proteins in the RA patients in comparison to that of the healthy controls. Two proteins (MW ∼32 kDa and ∼62 kDa) showed an alteration in expression while an altered monosaccharide profile (high mannose) was observed in the ∼62 kDa protein in the samples collected from RA patients. The 2-dimensional polyacrylamide gel electrophoresis analysis of the Con-A-bound plasma samples showed a large number of protein spots, a few of which were differentially expressed in the RA patients. Some unidentified proteins were detected in the RA patients which were absent in the control samples. The present study, therefore, enunciates the role of carbohydrates as well as that of the acute phase response in the disease pathogenesis.

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Authors and Affiliations

  1. Institute of Genomics & Integrative Biology, Delhi University Campus, Mall Road, Delhi, 110 007, India

    Sunil K. Raghav, Bhawna Gupta, Charu Agrawal, Ashish Saroha, Rakha H. Das & Hasi R. Das

  2. Department of Rheumatology, R&R, Army Hospital, N. Delhi, 110 010, India

    Ved P. Chaturvedi

Authors
  1. Sunil K. Raghav
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  2. Bhawna Gupta
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  3. Charu Agrawal
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  4. Ashish Saroha
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  5. Rakha H. Das
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  6. Ved P. Chaturvedi
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  7. Hasi R. Das
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Raghav, S.K., Gupta, B., Agrawal, C. et al. Altered expression and glycosylation of plasma proteins in rheumatoid arthritis. Glycoconj J 23, 167–173 (2006). https://doi.org/10.1007/s10719-006-7922-6

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  • DOI: https://doi.org/10.1007/s10719-006-7922-6

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