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Aneuploid progenies of triploid hybrids between diploid and tetraploid loach Misgurnus anguillicaudatus in China

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Abstract

Triploid Chinese loach, Misgurnus anguillicaudatus, hybrids between tetraploids from Hubei Province and diploids from Liaoning Province were mated with either diploid wild-type or triploid hybrids to analyze viability and ploidy of the resultant progenies. Both triploid males and females generated fertile gametes, but progenies from the crosses using gametes of triploid hybrids did not survive beyond the larval stages. In crosses between wild-type diploid females and triploid hybrid males, embryos ranging from 2.2n to 2.6n were predominant with a mode of either 2.4n (chromosome numbers 59, 60, 61) or 2.5n (chromosome numbers 62, 63). Those from the crosses between triploid hybrid females and diploid males gave a modal ploidy level at approximately 2.5n in one case, but a shift to a higher ploidy level was observed in other embryos. In the progenies between triploid hybrid females and males, the ploidy level at approximately 3.0n (chromosome numbers 74, 75, 76) was most frequent. The cytogenetic results of the progenies suggest the production of aneuploid gametes with a modal ploidy level at approximately 1.5n in triploid hybrids. However, a shift to higher chromosome numbers in gametes was observed in certain cases, suggesting the involvement of mortality selection of gametes and/or zygotes with lower chromosome numbers.

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Abbreviations

Ag-NORs:

silver staining nucleolus organizer regions.

CMA3 :

chromomycin A3.

CN:

chromosome number.

DA:

distamycin A.

DAPI:

4′6-diamidino-2-phenylindole.

FISH:

fluorescence in situ hybridization.

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Acknowledgments

This study was supported in part by KAKENHI Grant-in-Aid for Scientific Research (B) (No.21380114) from the Japan Society of the Promotion of Science (JSPS) to KA and Grants-in-Aid from the National Natural Science Foundation of China (No.31272650) and the Natural Science Foundation of Liaoning Province (No.201102019) to YJL.

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Authors and Affiliations

  1. Key Laboratory of Mariculture and Stock Enhancement in North China’s Sea, Ministry of Agriculture, Dalian Ocean University, Dalian, 116023, China

    Ya-Juan Li, He Zhou, Hai-Yan Ma, Zhong-Qiao Lin, Tian-Yu Ma & Yi Sui

  2. Research Center for Eco-Environmental Science, Chinese Academy of Science, 18 Shuangqing Road, Haidian District, Beijing, 100085, China

    Yang-Chun Gao

  3. Faculty and Graduate School of Fisheries Sciences, Hokkaido University, 3-1-1, Minato, Hakodate, Hokkaido, 041-8611, Japan

    Katsutoshi Arai

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  1. Ya-Juan Li
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  2. Yang-Chun Gao
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  3. He Zhou
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  4. Hai-Yan Ma
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  5. Zhong-Qiao Lin
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  6. Tian-Yu Ma
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  7. Yi Sui
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  8. Katsutoshi Arai
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Correspondence to Katsutoshi Arai.

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Li, YJ., Gao, YC., Zhou, H. et al. Aneuploid progenies of triploid hybrids between diploid and tetraploid loach Misgurnus anguillicaudatus in China. Genetica 144, 601–609 (2016). https://doi.org/10.1007/s10709-016-9928-8

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  • DOI: https://doi.org/10.1007/s10709-016-9928-8

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