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Fine mapping and genetic association analysis of Net2, the causative D-genome locus of low temperature-induced hybrid necrosis in interspecific crosses between tetraploid wheat and Aegilops tauschii

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Abstract

Hybrid necrosis has been observed in many interspecific hybrids from crosses between tetraploid wheat and the wheat D-genome donor Aegilops tauschii. Type II necrosis is a kind of hybrid incompatibility that is specifically characterized by low-temperature induction and growth suppression. Two complementary genes, Net1 on the AB genome and Net2 on the D genome, putatively control type II necrosis in ABD triploids and synthetic hexaploid wheat. Toward map-based cloning of Net2, a fine map around the Net2 region on 2DS was constructed in this study. Using the draft genome sequence of Ae. tauschii and the physical map of the barley genome, the Net2 locus was mapped within a 0.6 cM interval between two closely linked markers. Although local chromosomal rearrangements were observed in the Net2-corresponding region between the barley/Brachypodium and Ae. tauschii genomes, the two closely linked markers were significantly associated with type II necrosis in Ae. tauschii. These results suggest that these markers will aid efficient selection of Net2 non-carrier individuals from the Ae. tauschii population and intraspecific progeny, and could help with introgression of agriculturally important genes from Ae. tauschii to common wheat.

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Acknowledgments

We thank Dr. Yoshihiro Matsuoka for his providing of the genotyping data of the 83 Ae. tauschii accessions and Dr. Kentaro Yoshida for his critical reading of the manuscript. This work was supported by grants from the Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan (Grant-in-Aid for Scientific Research (B) Nos. 25292008 and 16H04862).

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  1. Graduate School of Agricultural Science, Kobe University, Rokkodai 1-1, Nada-ku, Kobe, 657-8501, Japan

    Kouhei Sakaguchi, Ryo Nishijima, Julio Cesar Masaru Iehisa & Shigeo Takumi

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  1. Kouhei Sakaguchi
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  2. Ryo Nishijima
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  3. Julio Cesar Masaru Iehisa
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  4. Shigeo Takumi
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Correspondence to Shigeo Takumi.

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Sakaguchi, K., Nishijima, R., Iehisa, J.C.M. et al. Fine mapping and genetic association analysis of Net2, the causative D-genome locus of low temperature-induced hybrid necrosis in interspecific crosses between tetraploid wheat and Aegilops tauschii . Genetica 144, 523–533 (2016). https://doi.org/10.1007/s10709-016-9920-3

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