Abstract
Nucleotide excision repair (NER) removes helix-distorting DNA lesions such as UV-induced pyrimidine dimers and cisplatin-induced strand crosslinking. Our earlier studies have identified low-molecular-weight proteins homologous to the 150-kDa vitellogenin 1 (Vg1) as UV-damaged DNA-binding factors expressed in developing zebrafish (Danio rerio). This present study explored if Vg1-like proteins also participated in NER in zebrafish. Immunoblot analysis of affinity-captured 12 h post-fertilization (hpf) zebrafish extract proteins showed a transient binding of a 30-kDa Vg1-like polypeptide to UV-damaged DNA. A transcription-based in vitro repair assay revealed a significant up-regulation of UVC or cisplatin-suppressed transcriptional activity of a marker cDNA driven by a SP6 RNA polymerase-regulated promotor after incubating the damaged plasmid with the extracts of 12 hpf embryos or 96 hpf larvae. The up-regulation of UV or cisplatin-suppressed transcription was abolished in the presence of a monoclonal anti-zebrafish Vg1 antibody. The differential sensitivity of UV-induced repair in 12 and 96 hpf zebrafish extracts to exogenous ATP suggested a development-dependent expression of Vg1-like NER factors. A T4 endonuclease V digestion assay showed no inhibition of the anti-Vg1 antibody on the excision of UV-induced cyclobutane pyrimidine dimers. Our results identified the participation of Vg1-like factors in NER in developing zebrafish, and these factors may function at post-incison steps of NER.
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This work was financially supported by the Ministry of Science and Technology of Taiwan, Republic of China, under grant numbers NSC 100-2313-B-019-009 and NSC 102-2313-B-019-017.
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Suppl. Fig.1.
Detection of T4 endonuclease V-mediated incision of UV-irradiated TT probe carrying a CPD by denaturing PAGE. Biotin-labelled duplex TT probe (4 pmole) was irradiated with UV at 9 kJ m-2 to generate a CPD between two adjacent TT bases on one strand according to a previous report (Pasheva et al. 1998). To confirm the production of CPD, UV-irradiated or unirradiated TT probe was incubated at 37 °C for 16 h with CPD-specific T4 endonuclease V(1 unit). The reaction mixture was electrophoresed on a 6% denaturing polyacrylamide gel and blotted overnight onto a nylon membrane. The presence of a biotin-labelled 5’ short single strand fragment (see arrow) due to enzyme-mediated incision and strand cleavage was detected by incubating the membrane with streptavidin-conjugated alkaline phosphatase and chemilluminesent enzyme substrates. (PPTX 124 kb)
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Shen, YC., Hsu, T., Ling, LB. et al. Identification of low-molecular-weight vitellogenin 1 (Vg1)-like proteins as nucleotide excision repair (NER) factors in developing zebrafish (Danio rerio) using a transcription-based DNA repair assay. Fish Physiol Biochem 43, 663–676 (2017). https://doi.org/10.1007/s10695-016-0321-4
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DOI: https://doi.org/10.1007/s10695-016-0321-4