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IRS1 and IRS2: molecular characterization, tissue expression and transcriptional regulation by insulin in yellow catfish Pelteobagrus fulvidraco

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Abstract

The insulin receptor substrate (IRS) proteins, in particular, IRS1 and IRS2, are the key downstream players of insulin signaling pathway and the regulation of lipid metabolism. In the present study, two genes of IRS (IRS1 and IRS2) were isolated and characterized from yellow catfish Pelteobagrus fulvidraco. Their molecular characterizations, tissue expressions, and transcriptional levels by insulin both in vivo and in vitro were determined. The validated complementary DNAs encoding for IRS1 and IRS2 were 3693 and 3177 bp in length, encoding proteins of 1230 and 1058 amino acid residues, respectively. Similarly to mammals, amino acid sequence alignment revealed that IRSs contained an N-terminal pleckstrin homology (PH) domain, a phosphotyrosine-binding (PTB) domain, and several C-terminal multiple sites of tyrosine phosphorylation. Both IRS1 and IRS2 were widely expressed across the ten tissues (liver, white muscle, spleen, brain, gill, mesenteric fat, anterior intestine, heart, mid-kidney, and ovary), but at the variable levels. Insulin injection at 1 μg/g in vivo significantly stimulated the messenger RNA (mRNA) expression of IRS2, but not IRS1 mRNA expression levels in the liver of yellow catfish after 48 h. In hepatocytes of yellow catfish, insulin incubation significantly stimulated the IRS1 (at a 1000 nM insulin group) and IRS2 (at both 100 and 1000 nM insulin groups) mRNA expressions, which indicated that IRS2 was more sensitive than IRS1 to insulin stimulation in the liver of yellow catfish, and IRS2 played a more important role in mediating insulin’s effects on the liver metabolism. The present study serves to increase our understanding into the function of IRS in fish.

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Abbreviations

GAPDH:

Glyceraldehyde-3-phosphate dehydrogenase

HPRT:

Hypoxanthine-guanine phosphoribosyl transferase

IRS:

Insulin receptor substrate

MS-222:

Tricaine methanesulfonate

MW:

Molecular weight

ORF:

Open-reading frame

PI3K:

Phosphoinositide 3-kiases

PH:

Pleckstrin homology

PTB:

Phosphotyrosine-binding

RPL7:

Ribosomal protein L7

SH2:

Src homology 2

TUBA:

Tubulin alpha chain

UBCE:

Ubiquitin-conjugating enzyme

UTR:

Untranslated region

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (Grant No. 31422056) and Fundamental Research Funds for the Central Universities, China (Grants Nos. 2014JQ002, 2662015PY017).

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Authors and Affiliations

  1. Key Laboratory of Freshwater Animal Breeding Ministry of Agriculture of P. R. C., Fishery College, Huazhong Agricultural University, Wuhan, 430070, China

    Mei-Qin Zhuo, Ya-Xiong Pan, Kun Wu, Yi-Huan Xu, Li-Han Zhang & Zhi Luo

  2. Freshwater Aquaculture Collaborative Innovative Centre of Hubei Province, Wuhan, 430070, China

    Mei-Qin Zhuo, Ya-Xiong Pan, Kun Wu, Yi-Huan Xu, Li-Han Zhang & Zhi Luo

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  1. Mei-Qin Zhuo
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  2. Ya-Xiong Pan
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  3. Kun Wu
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  4. Yi-Huan Xu
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  5. Li-Han Zhang
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  6. Zhi Luo
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Correspondence to Zhi Luo.

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Zhuo, MQ., Pan, YX., Wu, K. et al. IRS1 and IRS2: molecular characterization, tissue expression and transcriptional regulation by insulin in yellow catfish Pelteobagrus fulvidraco . Fish Physiol Biochem 43, 619–630 (2017). https://doi.org/10.1007/s10695-016-0316-1

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  • DOI: https://doi.org/10.1007/s10695-016-0316-1

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